VitelloTag: a tool for high-throughput cargo delivery into oocytes

VitelloTag: a tool for high-throughput cargo delivery into oocytes

Delivering molecular tools into oocytes is essential for developmental and reproductive biology. Microinjection, the conventional method, is equipment intensive, often technically challenging and has a low yield, and is impractical in species with delicate oocytes or restricted spawning seasons. To...

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Veröffentlicht in:Development (Cambridge) 2024-10, Vol.151 (20)
Hauptverfasser: Clarke, D Nathaniel, Kane, Akshay, Perillo, Margherita, Lowe, Christopher J, Swartz, S Zachary
Format: Artikel
Sprache:eng
Schlagworte:
Animals
CRISPR-Cas Systems - genetics
Endocytosis
Female
Gene Knockout Techniques
Microinjections - methods
Oocytes - metabolism
Techniques and Resources
Vitellogenins - genetics
Vitellogenins - metabolism
Zebrafish - embryology
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Zusammenfassung:Delivering molecular tools into oocytes is essential for developmental and reproductive biology. Microinjection, the conventional method, is equipment intensive, often technically challenging and has a low yield, and is impractical in species with delicate oocytes or restricted spawning seasons. To overcome these limitations, we developed VitelloTag, a cost-effective, high-throughput system using vitellogenin-derived fusion proteins to enable efficient cargo delivery via receptor-mediated endocytosis. We demonstrate its utility by delivering Cas9/sgRNA complexes in two distantly related species for gene knockout.
ISSN:0950-1991
1477-9129
1477-9129
DOI:10.1242/dev.202857