A denaturation-induced proton-uptake study of horse ferricytochrome c

A denaturation-induced proton-uptake study of horse ferricytochrome c

The observation that 6 M-urea denatures horse ferricytochrome c in the pH range 4-6, but not horse ferrocytochrome c, has been exploited to determine the denaturation-induced proton uptake of ferricytochrome c. This is related to the pKa values of ionizable groups buried within the native protein. T...

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Veröffentlicht in:Biochemical journal 1989-03, Vol.258 (2), p.595-598
Hauptverfasser: Hartshorn, R T, Moore, G R
Format: Artikel
Sprache:eng
Schlagworte:
Amino Acids
Animals
Cytochrome c Group - metabolism
Heme - metabolism
Horses
Hydrogen-Ion Concentration
Magnetic Resonance Spectroscopy
Propionates - metabolism
Protein Denaturation
Protons
Spectrometry, Fluorescence
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Zusammenfassung:The observation that 6 M-urea denatures horse ferricytochrome c in the pH range 4-6, but not horse ferrocytochrome c, has been exploited to determine the denaturation-induced proton uptake of ferricytochrome c. This is related to the pKa values of ionizable groups buried within the native protein. The data indicate that one of the haem propionic acid substituents of ferricytochrome c has a pKa of less than 4.5, whereas the other has a pKa of greater than 9.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj2580595