Incorporation of atmospheric oxygen into the carbonyl functionality of the protochlorophyllide isocyclic ring

Incorporation of atmospheric oxygen into the carbonyl functionality of the protochlorophyllide isocyclic ring

Detached cucumber (Cucumis sativus L. var. Beit Alpha) cotyledons incubated in darkness with 5-aminolaevulinic acid and either 16O2 air (control) or 18O2 in N2 accumulated protochlorophyllide. This was converted into methyl phaeoporphyrin alpha 5 and analysed by mass spectrometry. The molecular ion...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Biochemical journal 1989-01, Vol.257 (2), p.599-602
Hauptverfasser: Walker, C J, Mansfield, K E, Smith, K M, Castelfranco, P A
Format: Artikel
Sprache:eng
Schlagworte:
Atmosphere
Chlorophyll - analogs & derivatives
Mass Spectrometry
Oxygen - metabolism
Plants - metabolism
Protochlorophyllide - analogs & derivatives
Protochlorophyllide - metabolism
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Detached cucumber (Cucumis sativus L. var. Beit Alpha) cotyledons incubated in darkness with 5-aminolaevulinic acid and either 16O2 air (control) or 18O2 in N2 accumulated protochlorophyllide. This was converted into methyl phaeoporphyrin alpha 5 and analysed by mass spectrometry. The molecular ion of the methyl phaeoporphyrin alpha 5 derived from the 18O2 incubation was 2 mass units greater than that of the control, establishing that the oxo oxygen atom of the isocyclic ring is derived from atmospheric oxygen.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj2570599