On the mechanism of the increase in cardiolipin biosynthesis and resynthesis in hepatocytes during rat liver regeneration
CL (cardiolipin) is a major mitochondrial membrane phospholipid important for the regulation of mitochondrial function. We examined CL de novo biosynthesis and its resynthesis in isolated rat liver hepatocytes prepared 48 h subsequent to two-thirds PHx (partial hepatectomy). The pool size of CL and...
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| Veröffentlicht in: | Biochemical journal 2005-02, Vol.386 (Pt 1), p.137-143 |
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| creator | Webster, Jennifer Jiang, Jenny Y Lu, Biao Xu, Fred Y Taylor, William A Mymin, Mathew Zhang, Manna Minuk, Gerald Y Hatch, Grant M |
| description | CL (cardiolipin) is a major mitochondrial membrane phospholipid important for the regulation of mitochondrial function. We examined CL de novo biosynthesis and its resynthesis in isolated rat liver hepatocytes prepared 48 h subsequent to two-thirds PHx (partial hepatectomy). The pool size of CL and its de novo biosynthesis from [1,3-(3)H]glycerol were increased 3.3-fold (P |
| doi_str_mv | 10.1042/BJ20040655 |
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We examined CL de novo biosynthesis and its resynthesis in isolated rat liver hepatocytes prepared 48 h subsequent to two-thirds PHx (partial hepatectomy). The pool size of CL and its de novo biosynthesis from [1,3-(3)H]glycerol were increased 3.3-fold (P<0.05) and 3.1-fold (P<0.05) respectively in hepatocytes prepared from PHx rats compared with sham-operated controls. The reason for the increased CL biosynthesis was a 65% increase (P<0.05) in enzymic activity in PGP-S (phosphatidylglycerolphosphate synthase), a key enzyme in de novo CL biosynthesis. The increase in PGP-S activity was due to a 3-fold increase (P<0.05) of hepatic PGP-S mRNA expression. The increase in de novo CL biosynthesis and pool size corresponded to a 2.3-fold increase (P<0.05) in the amount of [1-14C]linoleic acid incorporated into CL of hepatocytes prepared from PHx rats compared with sham-operated controls, indicating an increase in CL resynthesis. The activity of MLCL-AT (monolysocardiolipin acyltransferase), a rate-limiting enzyme of CL resynthesis, was increased by 43% (P<0.05) in hepatocytes prepared from PHx rats compared with sham-operated controls; this result would explain the increase in [1-14C]linoleic acid incorporation into CL. The increase in MLCL-AT activity was due to an increase in hepatic MLCL-AT protein expression. The results show that CL de novo biosynthesis and its resynthesis are increased during liver regeneration.]]></description><identifier>ISSN: 0264-6021</identifier><identifier>EISSN: 1470-8728</identifier><identifier>DOI: 10.1042/BJ20040655</identifier><identifier>PMID: 15458384</identifier><language>eng</language><publisher>England: Portland Press Ltd</publisher><subject>Acyltransferases - analysis ; Animals ; Cardiolipins - biosynthesis ; Hepatectomy ; Hepatocytes - metabolism ; Liver Regeneration - physiology ; Mitochondria, Liver - enzymology ; Phospholipids - analysis ; Proliferating Cell Nuclear Antigen - analysis ; Proteins - analysis ; Rats ; Rats, Sprague-Dawley ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - biosynthesis ; Transferases (Other Substituted Phosphate Groups) - analysis</subject><ispartof>Biochemical journal, 2005-02, Vol.386 (Pt 1), p.137-143</ispartof><rights>The Biochemical Society, London 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c376t-8b864bdaec330d82ddd184b9d9ccb7e10551b93f278ea1b40f8328865a0046623</citedby><cites>FETCH-LOGICAL-c376t-8b864bdaec330d82ddd184b9d9ccb7e10551b93f278ea1b40f8328865a0046623</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1134775/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1134775/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15458384$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Webster, Jennifer</creatorcontrib><creatorcontrib>Jiang, Jenny Y</creatorcontrib><creatorcontrib>Lu, Biao</creatorcontrib><creatorcontrib>Xu, Fred Y</creatorcontrib><creatorcontrib>Taylor, William A</creatorcontrib><creatorcontrib>Mymin, Mathew</creatorcontrib><creatorcontrib>Zhang, Manna</creatorcontrib><creatorcontrib>Minuk, Gerald Y</creatorcontrib><creatorcontrib>Hatch, Grant M</creatorcontrib><title>On the mechanism of the increase in cardiolipin biosynthesis and resynthesis in hepatocytes during rat liver regeneration</title><title>Biochemical journal</title><addtitle>Biochem J</addtitle><description><![CDATA[CL (cardiolipin) is a major mitochondrial membrane phospholipid important for the regulation of mitochondrial function. We examined CL de novo biosynthesis and its resynthesis in isolated rat liver hepatocytes prepared 48 h subsequent to two-thirds PHx (partial hepatectomy). The pool size of CL and its de novo biosynthesis from [1,3-(3)H]glycerol were increased 3.3-fold (P<0.05) and 3.1-fold (P<0.05) respectively in hepatocytes prepared from PHx rats compared with sham-operated controls. The reason for the increased CL biosynthesis was a 65% increase (P<0.05) in enzymic activity in PGP-S (phosphatidylglycerolphosphate synthase), a key enzyme in de novo CL biosynthesis. The increase in PGP-S activity was due to a 3-fold increase (P<0.05) of hepatic PGP-S mRNA expression. The increase in de novo CL biosynthesis and pool size corresponded to a 2.3-fold increase (P<0.05) in the amount of [1-14C]linoleic acid incorporated into CL of hepatocytes prepared from PHx rats compared with sham-operated controls, indicating an increase in CL resynthesis. The activity of MLCL-AT (monolysocardiolipin acyltransferase), a rate-limiting enzyme of CL resynthesis, was increased by 43% (P<0.05) in hepatocytes prepared from PHx rats compared with sham-operated controls; this result would explain the increase in [1-14C]linoleic acid incorporation into CL. The increase in MLCL-AT activity was due to an increase in hepatic MLCL-AT protein expression. The results show that CL de novo biosynthesis and its resynthesis are increased during liver regeneration.]]></description><subject>Acyltransferases - analysis</subject><subject>Animals</subject><subject>Cardiolipins - biosynthesis</subject><subject>Hepatectomy</subject><subject>Hepatocytes - metabolism</subject><subject>Liver Regeneration - physiology</subject><subject>Mitochondria, Liver - enzymology</subject><subject>Phospholipids - analysis</subject><subject>Proliferating Cell Nuclear Antigen - analysis</subject><subject>Proteins - analysis</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Transferases (Other Substituted Phosphate Groups) - analysis</subject><issn>0264-6021</issn><issn>1470-8728</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkU1PGzEQhq2qqKS0l_6AyqceKi34284FCRBtQUhc6Nny2rOJq1072Buk_PsaiEh7mq9H74zmRegLJaeUCHZ2ecsIEURJ-Q4tqNCkM5qZ92hBmBKdIoweo4-1_iGEisZ9QMdUCmm4EQu0u094XgOewK9dinXCeXhpxOQLuPqcYO9KiHmMm5b3MdddakSNFbsUcIFD3eZr2Lg5-90MFYdtiWmFi5vxGJ-gNHYFCVodc_qEjgY3Vvi8jyfo94_rh6tf3d39z5uri7vOc63mzvRGiT448JyTYFgIgRrRL8PS-14DJVLSfskHpg042gsyGM6MUdK1nyjF-Ak6f9XdbPsJgoc0FzfaTYmTKzubXbT_T1Jc21V-spRyobVsAt_2AiU_bqHOdorVwzi6BHlbrdKCEMl0A7-_gr7kWgsMb0sosc9O2YNTDf7671kHdG8N_wv8DJGD</recordid><startdate>20050215</startdate><enddate>20050215</enddate><creator>Webster, Jennifer</creator><creator>Jiang, Jenny Y</creator><creator>Lu, Biao</creator><creator>Xu, Fred Y</creator><creator>Taylor, William A</creator><creator>Mymin, Mathew</creator><creator>Zhang, Manna</creator><creator>Minuk, Gerald Y</creator><creator>Hatch, Grant M</creator><general>Portland Press Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20050215</creationdate><title>On the mechanism of the increase in cardiolipin biosynthesis and resynthesis in hepatocytes during rat liver regeneration</title><author>Webster, Jennifer ; Jiang, Jenny Y ; Lu, Biao ; Xu, Fred Y ; Taylor, William A ; Mymin, Mathew ; Zhang, Manna ; Minuk, Gerald Y ; Hatch, Grant M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c376t-8b864bdaec330d82ddd184b9d9ccb7e10551b93f278ea1b40f8328865a0046623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Acyltransferases - analysis</topic><topic>Animals</topic><topic>Cardiolipins - biosynthesis</topic><topic>Hepatectomy</topic><topic>Hepatocytes - metabolism</topic><topic>Liver Regeneration - physiology</topic><topic>Mitochondria, Liver - enzymology</topic><topic>Phospholipids - analysis</topic><topic>Proliferating Cell Nuclear Antigen - analysis</topic><topic>Proteins - analysis</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Transferases (Other Substituted Phosphate Groups) - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Webster, Jennifer</creatorcontrib><creatorcontrib>Jiang, Jenny Y</creatorcontrib><creatorcontrib>Lu, Biao</creatorcontrib><creatorcontrib>Xu, Fred Y</creatorcontrib><creatorcontrib>Taylor, William A</creatorcontrib><creatorcontrib>Mymin, Mathew</creatorcontrib><creatorcontrib>Zhang, Manna</creatorcontrib><creatorcontrib>Minuk, Gerald Y</creatorcontrib><creatorcontrib>Hatch, Grant M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biochemical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Webster, Jennifer</au><au>Jiang, Jenny Y</au><au>Lu, Biao</au><au>Xu, Fred Y</au><au>Taylor, William A</au><au>Mymin, Mathew</au><au>Zhang, Manna</au><au>Minuk, Gerald Y</au><au>Hatch, Grant M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>On the mechanism of the increase in cardiolipin biosynthesis and resynthesis in hepatocytes during rat liver regeneration</atitle><jtitle>Biochemical journal</jtitle><addtitle>Biochem J</addtitle><date>2005-02-15</date><risdate>2005</risdate><volume>386</volume><issue>Pt 1</issue><spage>137</spage><epage>143</epage><pages>137-143</pages><issn>0264-6021</issn><eissn>1470-8728</eissn><abstract><![CDATA[CL (cardiolipin) is a major mitochondrial membrane phospholipid important for the regulation of mitochondrial function. We examined CL de novo biosynthesis and its resynthesis in isolated rat liver hepatocytes prepared 48 h subsequent to two-thirds PHx (partial hepatectomy). The pool size of CL and its de novo biosynthesis from [1,3-(3)H]glycerol were increased 3.3-fold (P<0.05) and 3.1-fold (P<0.05) respectively in hepatocytes prepared from PHx rats compared with sham-operated controls. The reason for the increased CL biosynthesis was a 65% increase (P<0.05) in enzymic activity in PGP-S (phosphatidylglycerolphosphate synthase), a key enzyme in de novo CL biosynthesis. The increase in PGP-S activity was due to a 3-fold increase (P<0.05) of hepatic PGP-S mRNA expression. The increase in de novo CL biosynthesis and pool size corresponded to a 2.3-fold increase (P<0.05) in the amount of [1-14C]linoleic acid incorporated into CL of hepatocytes prepared from PHx rats compared with sham-operated controls, indicating an increase in CL resynthesis. The activity of MLCL-AT (monolysocardiolipin acyltransferase), a rate-limiting enzyme of CL resynthesis, was increased by 43% (P<0.05) in hepatocytes prepared from PHx rats compared with sham-operated controls; this result would explain the increase in [1-14C]linoleic acid incorporation into CL. The increase in MLCL-AT activity was due to an increase in hepatic MLCL-AT protein expression. The results show that CL de novo biosynthesis and its resynthesis are increased during liver regeneration.]]></abstract><cop>England</cop><pub>Portland Press Ltd</pub><pmid>15458384</pmid><doi>10.1042/BJ20040655</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acyltransferases - analysis Animals Cardiolipins - biosynthesis Hepatectomy Hepatocytes - metabolism Liver Regeneration - physiology Mitochondria, Liver - enzymology Phospholipids - analysis Proliferating Cell Nuclear Antigen - analysis Proteins - analysis Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - biosynthesis Transferases (Other Substituted Phosphate Groups) - analysis |
| title | On the mechanism of the increase in cardiolipin biosynthesis and resynthesis in hepatocytes during rat liver regeneration |
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