Protein interaction quantified in vivo by spectrally resolved fluorescence resonance energy transfer

We describe a fluorescence resonance energy transfer (FRET)-based method for finding in living cells the fraction of a protein population (alpha(T)) forming complexes, and the average number (n) of those protein molecules in each complex. The method relies both on sensitized acceptor emission and on...

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Veröffentlicht in:	Biochemical journal 2005-01, Vol.385 (Pt 1), p.265-277
Hauptverfasser:	Raicu, Valerică, Jansma, David B, Miller, R J Dwayne, Friesen, James D
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Fluorescence Resonance Energy Transfer Green Fluorescent Proteins - chemistry Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism Luminescent Proteins - chemistry Luminescent Proteins - genetics Luminescent Proteins - metabolism Photobleaching Protein Binding Saccharomyces cerevisiae - genetics
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