Evaluation of the efficiency of entomopathogenic nematodes exposed to different temperatures of vinasse in the control of Stomoxys calcitrans (Linnaeus, 1758) (Diptera: Muscidae)

The sugarcane industry generates byproducts that contribute to the proliferation of . An analysis was carried out to verify the efficacy of HP88 and LPP7 at different vinasse temperatures to control larvae. Ten fly larvae were deposited in plastic containers containing four mL of 50% vinasse. Each treatment consisted of 300 EPN/larvae of added to the containers and heated at temperatures of 25, 28, 31, 34, 37 and 40 °C. The same treatments were performed using . The treatments were carried out in a BOD incubator at 25 ± 1 °C and 70 ± 10% RH, and each treatment was replicated six times. The treated groups, controls and temperatures showed no statistical differences in terms of larval mortality rate (P=0.8573), percentage of dead pupae (P=0.1782) and adult emergence (P=0.4386). Larval mortality rates of 30% and 14.17% were achieved with and , respectively, while the control groups presented 3.89% with and 8.61% with . From the standpoint of temperatures, significant differences were found only at 37 and 40 °C for . The highest pupal mortality achieved with was 34.17% at 31 °C, while that reached with at 37 °C was 40%. The groups containing caused lower adult emergence rates at temperatures of 25, 28, 31 and 34 °C, while caused its lowest emergence rates at 37 and 40 °C. It is concluded that infection occurs in the immature stages of by EPN when added to 50% vinasse solution at different temperatures and that nematodes caused negative effects on the emergence of fly larvae at varying temperatures.