Identification of the putative hexose-phosphate translocator of amyloplasts from cauliflower buds

Starch synthesis in amyloplasts isolated from cauliflower buds is strongly inhibited by the addition of micromolar concentrations of 4,4'-di-isothiocyano-2,2'-stilbenedisulphonic acid (DIDS). Using [3H]DIDS it was possible to label specifically a 31.6 kDa membrane protein of the envelope f...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Biochemical journal 1993-08, Vol.294 (1), p.15-17
Hauptverfasser:	BATZ, O, SCHEIBE, R, NEUHAUS, H. E
Format:	Artikel
Sprache:	eng
Schlagworte:	4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid - analogs & derivatives 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid - chemistry Analytical, structural and metabolic biochemistry Binding and carrier proteins Biological and medical sciences Brassica - metabolism Carrier Proteins - isolation & purification Carrier Proteins - metabolism Dihydroxyacetone Phosphate - pharmacology Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Glucose-6-Phosphate Glucosephosphates - pharmacology Organelles - metabolism Proteins Starch - biosynthesis
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Starch synthesis in amyloplasts isolated from cauliflower buds is strongly inhibited by the addition of micromolar concentrations of 4,4'-di-isothiocyano-2,2'-stilbenedisulphonic acid (DIDS). Using [3H]DIDS it was possible to label specifically a 31.6 kDa membrane protein of the envelope fraction of isolated amyloplasts. The intensity of the radioactive label was decreased in the presence of glucose 6-phosphate or dihydroxyacetone phosphate, indicating that this protein might be the amyloplastic hexosephosphate translocator.
ISSN:	0264-6021 1470-8728
DOI:	10.1042/bj2940015