Interleukin-1 activates transcription factor NFκB in glial cells

Recombinant human interleukin-1 (IL-1) alpha and beta were found to activate a latent cytosolic form of the transcription factor NF kappa B in rat C6 glioma. IL-1 beta was 10 times more potent than IL-1 alpha for this activity and both were inhibited by the IL-1 receptor antagonist. The activation w...

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Veröffentlicht in:Biochemical journal 1993-09, Vol.294 (2), p.343-347
Hauptverfasser: MOYNAGH, P. N, WILLIAMS, D. C, O'NEILL, L. A
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container_title Biochemical journal
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creator MOYNAGH, P. N
WILLIAMS, D. C
O'NEILL, L. A
description Recombinant human interleukin-1 (IL-1) alpha and beta were found to activate a latent cytosolic form of the transcription factor NF kappa B in rat C6 glioma. IL-1 beta was 10 times more potent than IL-1 alpha for this activity and both were inhibited by the IL-1 receptor antagonist. The activation was detectable from 20 min and remained sustained for up to 24 h. The electrophoretic mobility of the activated complex was shown to be different from that of the corresponding complexes in another IL-1-responsive cell line, the murine thymoma line EL4.NOB-1. C6 cells, when transiently transfected with five NF kappa B consensus sequence repeats linked to the reporter gene chloramphenicol acetyltransferase (CAT), demonstrated increased CAT activity in response to IL-1 beta treatment. The activation of NF kappa B in glial cells may thus represent an early step in IL-1 signalling in brain and is likely to have consequences for IL-1-induced gene expression in these cells.
doi_str_mv 10.1042/bj2940343
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C6 cells, when transiently transfected with five NF kappa B consensus sequence repeats linked to the reporter gene chloramphenicol acetyltransferase (CAT), demonstrated increased CAT activity in response to IL-1 beta treatment. The activation of NF kappa B in glial cells may thus represent an early step in IL-1 signalling in brain and is likely to have consequences for IL-1-induced gene expression in these cells.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cell physiology</subject><subject>Chloramphenicol O-Acetyltransferase - genetics</subject><subject>Consensus Sequence</subject><subject>Fundamental and applied biological sciences. 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source MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection
subjects Animals
Base Sequence
Biological and medical sciences
Cell physiology
Chloramphenicol O-Acetyltransferase - genetics
Consensus Sequence
Fundamental and applied biological sciences. Psychology
Gene Expression
Glioma
Humans
Interleukin-1 - pharmacology
Kinetics
Mice
Molecular and cellular biology
Molecular Sequence Data
NF-kappa B - genetics
NF-kappa B - metabolism
Rats
Recombinant Fusion Proteins - metabolism
Recombinant Proteins - pharmacology
Repetitive Sequences, Nucleic Acid
Signal transduction
Thymoma
Transfection
Tumor Cells, Cultured
title Interleukin-1 activates transcription factor NFκB in glial cells
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