In vitro selection of N1-methyladenosine-sensitive RNA-cleaving deoxyribozymes with 105-fold selectivity over unmethylated RNA

In vitro selection of N1-methyladenosine-sensitive RNA-cleaving deoxyribozymes with 105-fold selectivity over unmethylated RNA

RNA-cleaving DNAzymes (RCDs) are catalytically active DNA molecules that cleave a wide range of RNA targets with extremely high sequence-selectivity, but none is able to faithfully discriminate methylated from unmethylated RNA (typically

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Bibliographische Detailangaben
Veröffentlicht in:Chemical science (Cambridge) 2024-08, Vol.15 (33), p.13452-13458
Hauptverfasser: Shi, Jiarong, Zhang, Qiang, Wu, Yunping, Chang, Yangyang, Liu, Meng
Format: Artikel
Sprache:eng
Schlagworte:
Chemistry
Deoxyribonucleic acid
DNA
Gene expression
Real time
Ribonucleic acid
RNA
Selectivity
Synchronism
Time synchronization
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Beschreibung
Zusammenfassung:RNA-cleaving DNAzymes (RCDs) are catalytically active DNA molecules that cleave a wide range of RNA targets with extremely high sequence-selectivity, but none is able to faithfully discriminate methylated from unmethylated RNA (typically
ISSN:2041-6520
2041-6539
DOI:10.1039/d4sc02943g