Mobile-CRISPRi protocol optimization for Vibrionaceae
Mobile clustered regularly interspaced palindromic repeats interference (Mobile-CRISPRi) is an established method for bacterial gene expression knockdown. The deactivated Cas9 protein and guide RNA are isopropyl β-D-1-thiogalactopyranoside inducible, and all components are integrated into the chromo...
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| Veröffentlicht in: | Microbiology resource announcements 2024-07, Vol.13 (8), p.e0004024 |
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| creator | Geyman, Logan J Tanner, Madeline P Rosario-Meléndez, Natalia Peters, Jason M Mandel, Mark J van Kessel, Julia C |
| description | Mobile clustered regularly interspaced palindromic repeats interference (Mobile-CRISPRi) is an established method for bacterial gene expression knockdown. The deactivated Cas9 protein and guide RNA are isopropyl β-D-1-thiogalactopyranoside inducible, and all components are integrated into the chromosome via Tn
transposition. Here, we optimized methods specific for applying Mobile-CRISPRi in multiple
species. |
| doi_str_mv | 10.1128/mra.00040-24 |
| format | Article |
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| subjects | Microbial Genetics Protocols and Workflows |
| title | Mobile-CRISPRi protocol optimization for Vibrionaceae |
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