Mobile-CRISPRi protocol optimization for Vibrionaceae

Mobile-CRISPRi protocol optimization for Vibrionaceae

Mobile clustered regularly interspaced palindromic repeats interference (Mobile-CRISPRi) is an established method for bacterial gene expression knockdown. The deactivated Cas9 protein and guide RNA are isopropyl β-D-1-thiogalactopyranoside inducible, and all components are integrated into the chromo...

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Veröffentlicht in:Microbiology resource announcements 2024-07, Vol.13 (8), p.e0004024
Hauptverfasser: Geyman, Logan J, Tanner, Madeline P, Rosario-Meléndez, Natalia, Peters, Jason M, Mandel, Mark J, van Kessel, Julia C
Format: Artikel
Sprache:eng
Schlagworte:
Microbial Genetics
Protocols and Workflows
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Zusammenfassung:Mobile clustered regularly interspaced palindromic repeats interference (Mobile-CRISPRi) is an established method for bacterial gene expression knockdown. The deactivated Cas9 protein and guide RNA are isopropyl β-D-1-thiogalactopyranoside inducible, and all components are integrated into the chromosome via Tn transposition. Here, we optimized methods specific for applying Mobile-CRISPRi in multiple species.
ISSN:2576-098X
2576-098X
DOI:10.1128/mra.00040-24