3- and 4-phosphorylated phosphatidylinositols in the aquatic plant Spirodela polyrhiza L

Labelling of Spirodela polyrhiza L. plants with [3H]inositol and [32P]Pi yielded a series of phosphoinositides which were identified as PtdIns, PtdIns4P and PtdIns(4,5)P2. In addition, systematic degradation of a phospholipid extract identified PtdIns3P. Analysis of the distribution of 32P label bet...

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Veröffentlicht in:	Biochemical journal 1992-04, Vol.283 (1), p.255-260
Hauptverfasser:	Brearley, C.A, Hanke, D.E
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry aquatic plants Biological and medical sciences biosynthesis chemical composition Chromatography, High Pressure Liquid Freshwater Fundamental and applied biological sciences. Psychology Glycerolipids, phospholipids Glycerophosphates - analysis identification Inositol Phosphates - analysis Lipids Other biological molecules phosphates (esters) phosphatidylinositol 3-phosphate phosphatidylinositol 4-phosphate phosphatidylinositols Phosphatidylinositols - analysis phosphorus compounds Phosphorus Radioisotopes Phosphorylation plant metabolism Plants - chemistry Spirodela polyrhiza Tritium
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Zusammenfassung:	Labelling of Spirodela polyrhiza L. plants with [3H]inositol and [32P]Pi yielded a series of phosphoinositides which were identified as PtdIns, PtdIns4P and PtdIns(4,5)P2. In addition, systematic degradation of a phospholipid extract identified PtdIns3P. Analysis of the distribution of 32P label between the monoester and diester phosphate groups of PtdIns3P and PtdIns4P revealed differences in the labelling of the monoester phosphate, suggesting that the two PtdInsP species are not synthesized or metabolized in a co-ordinate manner.
ISSN:	0264-6021 1470-8728
DOI:	10.1042/bj2830255