p53 protein expression patterns associated with TP53 mutations in breast carcinoma
Purpose The importance of a TP53 mutation has been demonstrated in several tumor types, including breast cancer (BC). However, the accuracy of p53 protein expression as a predictor of gene mutation has not been well studied in BC. Therefore, we evaluated p53 protein expression associated with TP53 m...
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Veröffentlicht in: | Breast cancer research and treatment 2024-08, Vol.207 (1), p.213-222 |
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Sprache: | eng |
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Zusammenfassung: | Purpose
The importance of a
TP53
mutation has been demonstrated in several tumor types, including breast cancer (BC). However, the accuracy of p53 protein expression as a predictor of gene mutation has not been well studied in BC. Therefore, we evaluated p53 protein expression associated with
TP53
mutations in breast cancers from 64 patients.
Methods
TP53
mutation was examined using next-generation sequencing (NGS). p53 protein expression was examined using immunohistochemistry (IHC).
Results
Among the 64 BCs, 55% demonstrated abnormal expression patterns including 27% overexpression, 22% null, 6% equivocal with 45% having a wild-type pattern. A
TP53
mutation was present in 53% (34/64) of tumors including 30% (19/64) demonstrating a missense mutation, 11% (7/64) with a frameshift mutation, 11% (7/64) with a nonsense mutation, and 3% (1/64) with a splice site mutation. Abnormal expression of p53 protein was present in 33 of 34 (97%) tumors carrying a
TP53
mutation; conversely, a wild-type pattern was present in 28 of 30 (93%) tumors without a detectable mutation (
p
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ISSN: | 0167-6806 1573-7217 1573-7217 |
DOI: | 10.1007/s10549-024-07357-z |