X-Paste improves wound healing in diabetes via NF-E2-related factor/HO-1 signaling pathway
Diabetic foot ulcers (DFU), as severe complications of diabetes mellitus (DM), significantly compromise patient health and carry risks of amputation and mortality. To offer new insights into the occurrence and development of DFU, focusing on the therapeutic mechanisms of X-Paste (XP) of wound healin...
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Veröffentlicht in: | World journal of diabetes 2024-06, Vol.15 (6), p.1299-1316 |
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Zusammenfassung: | Diabetic foot ulcers (DFU), as severe complications of diabetes mellitus (DM), significantly compromise patient health and carry risks of amputation and mortality.
To offer new insights into the occurrence and development of DFU, focusing on the therapeutic mechanisms of X-Paste (XP) of wound healing in diabetic mice.
Employing traditional Chinese medicine ointment preparation methods, XP combines various medicinal ingredients. High-performance liquid chromatography (HPLC) identified XP's main components. Using streptozotocin (STZ)-induced diabetic, we aimed to investigate whether XP participated in the process of diabetic wound healing. RNA-sequencing analyzed gene expression differences between XP-treated and control groups. Molecular docking clarified XP's treatment mechanisms for diabetic wound healing. Human umbilical vein endothelial cells (HUVECs) were used to investigate the effects of Andrographolide (Andro) on cell viability, reactive oxygen species generation, apoptosis, proliferation, and metastasis
following exposure to high glucose (HG), while NF-E2-related factor-2 (
) knockdown elucidated Andro's molecular mechanisms.
XP notably enhanced wound healing in mice, expediting the healing process. RNA-sequencing revealed
upregulation in DM tissues following XP treatment. HPLC identified 21 primary XP components, with Andro exhibiting strong
binding. Andro mitigated HG-induced HUVECs proliferation, metastasis, angiogenic injury, and inflammation inhibition. Andro alleviates HG-induced HUVECs damage through
/HO-1 pathway activation, with
knockdown reducing Andro's proliferative and endothelial protective effects.
XP significantly promotes wound healing in STZ-induced diabetic models. As XP's key component, Andro activates the
/HO-1 signaling pathway, enhancing cell proliferation, tubule formation, and inflammation reduction. |
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ISSN: | 1948-9358 1948-9358 |
DOI: | 10.4239/wjd.v15.i6.1299 |