Phenotypic identification of different β-Lactamases in intrinsic and acquired colistin resistant uropathogenic gram negative bacteria

Identification of MBL, AmpC and ESBLs in colistin intrinsic and acquired resistant uropathogenic gram negative bacteria. Urine samples were collected from Hayatabad Medical Complex, Peshawar during 17 January to 30 June 2019. Collected urine samples were aseptically transported microbiology lab of Health Research Institution (HRI), National Institute of Health (NIH), Khyber Medical College, Peshawar and streaked on different media. Positive growth was identified by API-10s. Antibiotic sensitivity profile was done by Modified Kirby Bauer disc diffusion method. Detection of metallo βlactamases (MBL) production by Imipenem EDTA synergy test, Double Disc Synergy Test (DDST) for detection of ESBLs and D-test for the detection of inducible AmpC beta lactamases test was used. Colistin resistance was identified via broth micro dilution according to CLSI manual. Colistin resistant bacteria was divided in two categories; acquired and intrinsic resistant bacteria according to CLSI manual. Out of 2000 urine samples, 281(14%) gram-negative bacteria were isolated. Among positive samples, acquired colistin resistant bacteria were 241 and intrinsic resistant bacteria were 40 isolates. MBL was produce by twenty one (11.7%) and seventeen (40.5 and were ESBLs producing bacteria. AmpC production was prevalent in fourteen (7.8%) and twelve (28.6 . Fifty-five samples showed resistance to colistin out of 241 samples. In colistin resistant bacteria, two were MBL, ESBLs, while one was ESBLs, AmpC co-producing bacteria. The most prevalent extended drug resistant bacteria were 6.1%), While 155(86.6%) , 25 (59.5%) and 22 (95.7%) was multi drug resistant bacteria. Current study concluded that ESBL, MBL AmpC enzymes and their co-expression was observed with colistin resistance in and .