In vitro Biological Properties and in Silico Studies on Tinospora Cordifolia Stem Aqueous Extract

, commonly known as "Giloy" or "Guduchi," is a medicinal plant with a rich history in traditional medicine systems. The aqueous extract of stems has garnered attention due to its reported pharmacological activities. This study aimed to investigate the biological properties of the aqueous extract and complement the findings with studies to gain insights into potential molecular interactions. The stem aqueous extract was subjected to a battery of assays to assess its biological properties. Anti-inflammatory activity was evaluated using invitro assay. To complement the in vitro findings, in silico studies involving molecular docking analyses were conducted to predict potential interactions between the extract's constituents and relevant biomolecular targets. The in vitro evaluation revealed significant anti-inflammatory activity of the Tinospora cordifolia stem aqueous extract, as evidenced by its ability to suppress the production of pro-inflammatory cytokines. In silico studies provided insights into the molecular interactions between the extract's bioactive constituents and key inflammatory and antioxidant targets, further supporting the observed biological properties. The combined in vitro biological assays and in silico studies offer a comprehensive assessment of the Tinospora cordifolia stem aqueous extract's potential therapeutic properties. The demonstrated anti-inflammatory activities align with the traditional use of Tinospora cordifolia and suggest its potential in managing inflammatory and oxidative stress-related disorders. The in silico insights provide a molecular understanding of the extract's mode of action, strengthening the rationale for further investigation and development of natural products derived from Tinospora cordifolia for pharmaceutical and medicinal applications.