Photoperiod-1 regulates the wheat inflorescence transcriptome to influence spikelet architecture and flowering time

Photoperiod insensitivity has been selected by breeders to help adapt crops to diverse environments and farming practices. In wheat, insensitive alleles of Photoperiod-1 (Ppd-1) relieve the requirement of long daylengths to flower by promoting expression of floral promoting genes early in the season; however, these alleles also limit yield by reducing the number and fertility of grain-producing florets through processes that are poorly understood. Here, we performed transcriptome analysis of the developing inflorescence using near-isogenic lines that contain either photoperiod-insensitive or null alleles of Ppd-1, during stages when spikelet number is determined and floret development initiates. We report that Ppd-1 influences the stage-specific expression of genes with roles in auxin signaling, meristem identity, and protein turnover, and analysis of differentially expressed transcripts identified bZIP and ALOG transcription factors, namely PDB1 and ALOG1, which regulate flowering time and spikelet architecture. These findings enhance our understanding of genes that regulate inflorescence development and introduce new targets for improving yield potential. [Display omitted] •Ppd-1 significantly influences the transcriptome of a developing wheat inflorescence•PDB1 regulates photoperiod-insensitive flowering and spikelet termination•ALOG1 suppresses spikelet branching and delays flowering•ALOG1 shares a conserved role in the Triticeae crops wheat and barley Gauley et al. investigate the influence of Photoperiod-1 (Ppd-1) during early wheat inflorescence development and identify two transcription factors that are regulated by Ppd-1 to control flowering time and inflorescence architecture.