Detecting organelle-specific activity of potassium channels with a DNA nanodevice

Cell surface potassium ion (K + ) channels regulate nutrient transport, cell migration and intercellular communication by controlling K + permeability and are thought to be active only at the plasma membrane. Although these channels transit the trans -Golgi network, early and recycling endosomes, whether they are active in these organelles is unknown. Here we describe a pH-correctable, ratiometric reporter for K + called pHlicKer, use it to probe the compartment-specific activity of a prototypical voltage-gated K + channel, Kv11.1, and show that this cell surface channel is active in organelles. Lumenal K + in organelles increased in cells expressing wild-type Kv11.1 channels but not after treatment with current blockers. Mutant Kv11.1 channels, with impaired transport function, failed to increase K + levels in recycling endosomes, an effect rescued by pharmacological correction. By providing a way to map the organelle-specific activity of K + channels, pHlicKer technology could help identify new organellar K + channels or channel modulators with nuanced functions. A potassium activity reporter detects organelle-specific channel function.