Poultry Gastrointestinal-derived Lactic Acid Bacteria (pGIT-d-LAB) Inhibit Multiple Antibiotics Resistance Bacterial and Fungal Pathogens

To develop a probiotic formulation for poultry feed, a few poultry gastrointestinal derived lactic acid bacteria (pGIT-d-LAB) were isolated from chicken intestinal specimens and experiment was performed to evaluate their efficacy as potential probiotic candidate. A total of 6 strains of LAB: ( ), ( ), ( ), spp, ( ) and ( ) were isolated and cultured for collection of Cell Free Supernatant (CFS). CFS collected was tested against pathogenic bacterial isolated from chicken feces as well as prevalent fungal pathogens, utilizing agar-well diffusion techniques. A preliminary investigation into the susceptibility of the pathogens to diverse antibiotics and antifungal drugs was conducted. Bacterial pathogens exhibiting resistance to a minimum of three classes of antibiotics were subsequently identified for pGIT-d-LAB CFS screening. The observed results revealed that the CFS derived from the isolates exhibited varying degrees of growth inhibition against different pathogens. Among the tested pGIT-d-LAB isolates, demonstrated the most prominent zone of inhibition, measuring 18 against ZTAC 1233. Notably, ZTAC 1255 showed resistance to all tested pGIT-d-LAB. Quantification of the metabolites produced was performed, and peak production levels was determined. produced the highest amount of lactic acid (1.789 ), spp. produced the highest amount of diacetyl and H 0 (1.918 ) (0.0025 ) at 48 peak values respectively. The test isolates are potential probiotic candidates for controlling pathogens in poultry.