Quartz crystal microbalance–based aptasensor integrated with magnetic pre-concentration system for detection of Listeria monocytogenes in food samples

A fast and accurate identification of Listeria monocytogenes. A new quartz crystal microbalance (QCM) aptasensor was designed for the specific and rapid detection of L. monocytogenes . Before detection of the target bacterium from samples in the QCM aptasensor, a magnetic pre-enrichment system was u...

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Veröffentlicht in:Mikrochimica acta (1966) 2024-05, Vol.191 (5), p.235-235, Article 235
Hauptverfasser: Beyazit, Fatma, Arica, Mehmet Yakup, Acikgoz-Erkaya, Ilkay, Ozalp, Cengiz, Bayramoglu, Gulay
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Sprache:eng
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Zusammenfassung:A fast and accurate identification of Listeria monocytogenes. A new quartz crystal microbalance (QCM) aptasensor was designed for the specific and rapid detection of L. monocytogenes . Before detection of the target bacterium from samples in the QCM aptasensor, a magnetic pre-enrichment system was used to eliminate any contaminant in the samples. The prepared magnetic system was characterized using ATR-FTIR, SEM, VSM, BET, and analytical methods. The saturation magnetization values of the Fe 3 O 4 , Fe 3 O 4 @PDA, and Fe 3 O 4 @PDA@DAPEG particles were 57.2, 40.8, and 36.4 emu/g, respectively. The same aptamer was also immobilized on the QCM crystal integrated into QCM flow cell and utilized to quantitatively detect L. monocytogenes cells from the samples. It was found that a specific aptamer-magnetic pre-concentration system efficiently captured L. monocytogenes cells in a short time (approximately 10 min). The Fe 3 O 4 @PDA@DA-PEG-Apt particles provided selective isolation of L. monocytogenes from the bacteria-spiked media up to 91.8%. The immobilized aptamer content of the magnetic particles was 5834 µg/g using 500 ng Apt/mL. The QCM aptasensor showed a very high range of analytical performance to the target bacterium from 1.0 × 10 2 and 1.0 × 10 7 CFU/mL. The limit of detection (LOD) and limit of quantitation (LOQ) were 148 and 448 CFU/mL, respectively, from the feeding of the QCM aptasensor flow cell with the eluent of the magnetic pre-concentration system. The reproducibility of the aptasensor was more than 95%. The aptasensor was very specific to L. monocytogenes compared to the other Listeria species (i.e., L . ivanovii , L . innocua , and  L. seeligeri ) or other tested bacteria such as  Staphylococcus aureus , Escherichia coli , and  Bacillus subtilis . The QCM aptasensor was regenerated with NaOH solution, and the system was reused many times. Graphical Abstract
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-024-06307-2