Alcohol use disorder (AUD) is associated with enhanced sensitivity to cellular lipopolysaccharide challenge
Inflammation has been associated with alcohol use disorder (AUD). A novel method to characterize AUD-related immune signaling involves probing Toll-like receptor (TLR)-4 stimulated monocyte production of intracellular cytokines (ICCs) via lipopolysaccharide (LPS). We evaluated relationships between...
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Veröffentlicht in: | Alcohol, clinical & experimental research clinical & experimental research, 2023-10, Vol.47 (10), p.1859-1868 |
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Sprache: | eng |
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Zusammenfassung: | Inflammation has been associated with alcohol use disorder (AUD). A novel method to characterize AUD-related immune signaling involves probing Toll-like receptor (TLR)-4 stimulated monocyte production of intracellular cytokines (ICCs) via lipopolysaccharide (LPS). We evaluated relationships between AUD and ICC production at rest and after LPS stimulation.
We analyzed blood samples from 36 participants (AUD N = 14; Controls N = 22), collected across time, with ICC expression assessed at rest (i.e., unstimulated) and following stimulation with LPS (i.e., a total of 5 repeated unstimulated or stimulated measures/participant). Markers assessed included tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), TNF-α and IL-6 co-expression, and interferon (IFN). For each marker, we constructed linear mixed models with AUD, LPS, and timepoint as fixed effects (BMI as covariate), allowing for random slope and intercept. AUD × LPS was included as an interaction.
For TLR4-stimulated monocyte production of TNF-α, there were effects of AUD (p |
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ISSN: | 2993-7175 0145-6008 2993-7175 1530-0277 |
DOI: | 10.1111/acer.15173 |