Comparison of the Cat2 complementary DNA sequences of a normal catalase activity line (W64A) and a high catalase activity line (R6-67) of maize

We have cloned and sequenced the Cat2 cDNA from maize inbred line W64A (the typical catalase activity line). Polyadenylated RNA was isolated from 2-d-old W64A scutella. A cDNA library was constructed in the lambda -Zap vector with the double-stranded cDNA. The library was plated on EScherichia coli...

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Veröffentlicht in:	Plant physiology (Bethesda) 1991-08, Vol.96 (4), p.1379-1381
Hauptverfasser:	Guan, L. (North Carolina State University, Raleigh, NC), Ruzsa, S, Skadsen, R.W, Scandalios, J.G
Format:	Artikel
Sprache:	eng
Schlagworte:	ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE AMINO ACID SEQUENCES Amino acids Biological and medical sciences CATALASA CATALASE CODE GENETIQUE CODIGO GENETICO Complementary DNA Corn embl x54819 Enzymes ENZYMIC ACTIVITY EXPRESION GENICA EXPRESSION DES GENES Fundamental and applied biological sciences. Psychology GENE EXPRESSION Gene expression regulation Genes Genes. Genome GENETIC ANALYSIS GENETIC CODE GENETICA GENETICS GENETIQUE INBRED LINES LIGNEE CONSANGUINE LINEAS CONSANGUINEAS Molecular and cellular biology Molecular Biology and Gene Regulation Molecular genetics molecular sequence data NUCLEOTIDE NUCLEOTIDES NUCLEOTIDOS Plant Gene Register Sequencing ZEA MAYS
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Beschreibung
Zusammenfassung:	We have cloned and sequenced the Cat2 cDNA from maize inbred line W64A (the typical catalase activity line). Polyadenylated RNA was isolated from 2-d-old W64A scutella. A cDNA library was constructed in the lambda -Zap vector with the double-stranded cDNA. The library was plated on EScherichia coli BB4 and screened with the Cat2 gene-specific probe. The positive clone was further subcloned into Bluescript vector and sequenced.
ISSN:	0032-0889 1532-2548
DOI:	10.1104/pp.96.4.1379