RiPP enzyme heterocomplex structure-guided discovery of a bacterial borosin α- N -methylated peptide natural product
Amide peptide backbone methylation is a characteristic post-translational modification found in a family of ribosomally synthesized and post-translationally modified peptide natural products (RiPPs) called borosins. Previously, we bioinformatically identified >1500 putative borosin pathways in ba...
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Veröffentlicht in: | RSC chemical biology 2023-10, Vol.4 (10), p.804-816 |
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Sprache: | eng |
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Zusammenfassung: | Amide peptide backbone methylation is a characteristic post-translational modification found in a family of ribosomally synthesized and post-translationally modified peptide natural products (RiPPs) called borosins. Previously, we bioinformatically identified >1500 putative borosin pathways in bacteria; however, none of the pathways were associated with a known secondary metabolite. Through in-depth characterization of a borosin pathway in
MR-1, we have now identified a bacterially derived borosin natural product named Shewanellamide A. Borosin identification was facilitated by the creation and analysis of a series of precursor variants and crystallographic interrogation of variant precursor and methyltransferase complexes. Along with assaying two proteases from
, probable boundaries for proteolytic maturation of the metabolite were projected and confirmed
comparison of
knockout and overexpression strains. All in all, the
natural product was found to be a 16-mer linear peptide featuring two backbone methylations, establishing Shewanellamide A as one of the few borosin metabolites yet identified, and the first from bacteria. |
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ISSN: | 2633-0679 2633-0679 |
DOI: | 10.1039/d3cb00093a |