Molecular procedure for rapid detection of Burkholderia mallei and Burkholderia pseudomallei

A PCR procedure for the discrimination of Burkholderia mallei and Burkholderia pseudomallei was developed. It is based on the nucleotide difference T 2143 C (T versus C at position 2143) between B. mallei and B. pseudomallei detected in the 23S rDNA sequences. In comparison with conventional methods...

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Veröffentlicht in:	Journal of clinical microbiology 1998-09, Vol.36 (9), p.2737-2741
Hauptverfasser:	BAUERNFEIND, A, ROLLER, C, MEYER, D, JUNGWIRTH, R, SCHNEIDER, I
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriological methods and techniques used in bacteriology Bacteriology Base Sequence Biological and medical sciences Burkholderia - classification Burkholderia - genetics Burkholderia - isolation & purification Burkholderia Infections - diagnosis Burkholderia Infections - prevention & control Burkholderia Infections - transmission Burkholderia pseudomallei - classification Burkholderia pseudomallei - genetics Burkholderia pseudomallei - isolation & purification DNA Primers DNA, Ribosomal - chemistry DNA, Ribosomal - genetics DNA, Ribosomal - isolation & purification Electrophoresis, Agar Gel Fundamental and applied biological sciences. Psychology Genetic Variation Humans Medical Laboratory Personnel Melioidosis - diagnosis Melioidosis - prevention & control Melioidosis - transmission Microbiology Molecular Sequence Data Nucleic Acid Conformation Occupational Diseases - microbiology Occupational Diseases - prevention & control Polymerase Chain Reaction - methods RNA, Ribosomal, 23S - genetics Sensitivity and Specificity Sequence Alignment Sequence Homology, Nucleic Acid
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Beschreibung
Zusammenfassung:	A PCR procedure for the discrimination of Burkholderia mallei and Burkholderia pseudomallei was developed. It is based on the nucleotide difference T 2143 C (T versus C at position 2143) between B. mallei and B. pseudomallei detected in the 23S rDNA sequences. In comparison with conventional methods the procedure allows more rapid identification at reduced risk for infection of laboratory personnel.
ISSN:	0095-1137 1098-660X
DOI:	10.1128/jcm.36.9.2737-2741.1998