Femtosecond proton transfer in urea solutions probed by X-ray spectroscopy

Proton transfer is one of the most fundamental events in aqueous-phase chemistry and an emblematic case of coupled ultrafast electronic and structural dynamics 1 , 2 . Disentangling electronic and nuclear dynamics on the femtosecond timescales remains a formidable challenge, especially in the liquid phase, the natural environment of biochemical processes. Here we exploit the unique features of table-top water-window X-ray absorption spectroscopy 3 – 6 to reveal femtosecond proton-transfer dynamics in ionized urea dimers in aqueous solution. Harnessing the element specificity and the site selectivity of X-ray absorption spectroscopy with the aid of ab initio quantum-mechanical and molecular-mechanics calculations, we show how, in addition to the proton transfer, the subsequent rearrangement of the urea dimer and the associated change of the electronic structure can be identified with site selectivity. These results establish the considerable potential of flat-jet, table-top X-ray absorption spectroscopy 7 , 8 in elucidating solution-phase ultrafast dynamics in biomolecular systems. The potential of water-window X-ray absorption spectroscopy for disentangling individual aspects of the respective electronic and structural dynamics in ultrafast non-adiabatic dynamics of molecular systems in a liquid environment is established.