Determination of 24,25-Dihydroxyvitamin D3 [24,25(OH)2D3] in Archived Vitamin D External Quality Assessment Scheme (DEQAS) Samples using a Reference Measurement Procedure with Comparison to DEQAS Participant Results and Assessment of Contribution of 24,25(OH)2D3 to DEQAS Assay Results

Ninety archived human serum samples from the Vitamin D External Quality Assessment Scheme (DEQAS) were analyzed using a reference measurement procedure (RMP) based on isotope dilution liquid chromatography – tandem mass spectrometry (ID LC-MS/MS) for the determination of 24,25-dihydroxyvitamin D 3 [...

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Veröffentlicht in:The Journal of steroid biochemistry and molecular biology 2023-05, Vol.231, p.106318-106318
Hauptverfasser: Wise, Stephen A., Hahm, Grace, Burdette, Carolyn Q., Tai, Susan S.-C., Camara, Johanna E., Sempos, Christopher T., Williams, Emma L.
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container_issue
container_start_page 106318
container_title The Journal of steroid biochemistry and molecular biology
container_volume 231
creator Wise, Stephen A.
Hahm, Grace
Burdette, Carolyn Q.
Tai, Susan S.-C.
Camara, Johanna E.
Sempos, Christopher T.
Williams, Emma L.
description Ninety archived human serum samples from the Vitamin D External Quality Assessment Scheme (DEQAS) were analyzed using a reference measurement procedure (RMP) based on isotope dilution liquid chromatography – tandem mass spectrometry (ID LC-MS/MS) for the determination of 24,25-dihydroxyvitamin D 3 [24,25(OH) 2 D 3 ]. These 24,25(OH) 2 D 3 results, in conjunction with concentration values assigned using RMPs for 25-hydroxyvitamin D 2 [25(OH)D 2 ] and 25-hydroxyvitamin D 3 [25(OH)D 3 ], provide a valuable resource for assessing the accuracy of measurements for 24,25(OH) 2 D 3 and for investigating the relationship between 24,25(OH) 2 D 3 and 25(OH)D 3 . Results for 24,25(OH) 2 D 3 using the RMP were compared to DEQAS consensus values demonstrating that the consensus values were not sufficient to assess the accuracy of measurements among different laboratories and methods. A multivariable regression analysis approach using historical DEQAS consensus values for various total 25(OH)D assays was used to assess the contribution of 24,25(OH) 2 D 3 concentration on the assay response. The response of several ligand binding assays for total 25(OH)D was shown to be impacted by the presence of 24,25(OH) 2 D 3 .
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title Determination of 24,25-Dihydroxyvitamin D3 [24,25(OH)2D3] in Archived Vitamin D External Quality Assessment Scheme (DEQAS) Samples using a Reference Measurement Procedure with Comparison to DEQAS Participant Results and Assessment of Contribution of 24,25(OH)2D3 to DEQAS Assay Results
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