Determination of 24,25-Dihydroxyvitamin D3 [24,25(OH)2D3] in Archived Vitamin D External Quality Assessment Scheme (DEQAS) Samples using a Reference Measurement Procedure with Comparison to DEQAS Participant Results and Assessment of Contribution of 24,25(OH)2D3 to DEQAS Assay Results
Ninety archived human serum samples from the Vitamin D External Quality Assessment Scheme (DEQAS) were analyzed using a reference measurement procedure (RMP) based on isotope dilution liquid chromatography – tandem mass spectrometry (ID LC-MS/MS) for the determination of 24,25-dihydroxyvitamin D 3 [...
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Veröffentlicht in: | The Journal of steroid biochemistry and molecular biology 2023-05, Vol.231, p.106318-106318 |
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container_title | The Journal of steroid biochemistry and molecular biology |
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creator | Wise, Stephen A. Hahm, Grace Burdette, Carolyn Q. Tai, Susan S.-C. Camara, Johanna E. Sempos, Christopher T. Williams, Emma L. |
description | Ninety archived human serum samples from the Vitamin D External Quality Assessment Scheme (DEQAS) were analyzed using a reference measurement procedure (RMP) based on isotope dilution liquid chromatography – tandem mass spectrometry (ID LC-MS/MS) for the determination of 24,25-dihydroxyvitamin D
3
[24,25(OH)
2
D
3
]. These 24,25(OH)
2
D
3
results, in conjunction with concentration values assigned using RMPs for 25-hydroxyvitamin D
2
[25(OH)D
2
] and 25-hydroxyvitamin D
3
[25(OH)D
3
], provide a valuable resource for assessing the accuracy of measurements for 24,25(OH)
2
D
3
and for investigating the relationship between 24,25(OH)
2
D
3
and 25(OH)D
3
. Results for 24,25(OH)
2
D
3
using the RMP were compared to DEQAS consensus values demonstrating that the consensus values were not sufficient to assess the accuracy of measurements among different laboratories and methods. A multivariable regression analysis approach using historical DEQAS consensus values for various total 25(OH)D assays was used to assess the contribution of 24,25(OH)
2
D
3
concentration on the assay response. The response of several ligand binding assays for total 25(OH)D was shown to be impacted by the presence of 24,25(OH)
2
D
3
. |
doi_str_mv | 10.1016/j.jsbmb.2023.106318 |
format | Article |
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3
[24,25(OH)
2
D
3
]. These 24,25(OH)
2
D
3
results, in conjunction with concentration values assigned using RMPs for 25-hydroxyvitamin D
2
[25(OH)D
2
] and 25-hydroxyvitamin D
3
[25(OH)D
3
], provide a valuable resource for assessing the accuracy of measurements for 24,25(OH)
2
D
3
and for investigating the relationship between 24,25(OH)
2
D
3
and 25(OH)D
3
. Results for 24,25(OH)
2
D
3
using the RMP were compared to DEQAS consensus values demonstrating that the consensus values were not sufficient to assess the accuracy of measurements among different laboratories and methods. A multivariable regression analysis approach using historical DEQAS consensus values for various total 25(OH)D assays was used to assess the contribution of 24,25(OH)
2
D
3
concentration on the assay response. The response of several ligand binding assays for total 25(OH)D was shown to be impacted by the presence of 24,25(OH)
2
D
3
.</description><identifier>ISSN: 0960-0760</identifier><identifier>EISSN: 1879-1220</identifier><identifier>DOI: 10.1016/j.jsbmb.2023.106318</identifier><identifier>PMID: 37169270</identifier><language>eng</language><ispartof>The Journal of steroid biochemistry and molecular biology, 2023-05, Vol.231, p.106318-106318</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids></links><search><creatorcontrib>Wise, Stephen A.</creatorcontrib><creatorcontrib>Hahm, Grace</creatorcontrib><creatorcontrib>Burdette, Carolyn Q.</creatorcontrib><creatorcontrib>Tai, Susan S.-C.</creatorcontrib><creatorcontrib>Camara, Johanna E.</creatorcontrib><creatorcontrib>Sempos, Christopher T.</creatorcontrib><creatorcontrib>Williams, Emma L.</creatorcontrib><title>Determination of 24,25-Dihydroxyvitamin D3 [24,25(OH)2D3] in Archived Vitamin D External Quality Assessment Scheme (DEQAS) Samples using a Reference Measurement Procedure with Comparison to DEQAS Participant Results and Assessment of Contribution of 24,25(OH)2D3 to DEQAS Assay Results</title><title>The Journal of steroid biochemistry and molecular biology</title><description>Ninety archived human serum samples from the Vitamin D External Quality Assessment Scheme (DEQAS) were analyzed using a reference measurement procedure (RMP) based on isotope dilution liquid chromatography – tandem mass spectrometry (ID LC-MS/MS) for the determination of 24,25-dihydroxyvitamin D
3
[24,25(OH)
2
D
3
]. These 24,25(OH)
2
D
3
results, in conjunction with concentration values assigned using RMPs for 25-hydroxyvitamin D
2
[25(OH)D
2
] and 25-hydroxyvitamin D
3
[25(OH)D
3
], provide a valuable resource for assessing the accuracy of measurements for 24,25(OH)
2
D
3
and for investigating the relationship between 24,25(OH)
2
D
3
and 25(OH)D
3
. Results for 24,25(OH)
2
D
3
using the RMP were compared to DEQAS consensus values demonstrating that the consensus values were not sufficient to assess the accuracy of measurements among different laboratories and methods. A multivariable regression analysis approach using historical DEQAS consensus values for various total 25(OH)D assays was used to assess the contribution of 24,25(OH)
2
D
3
concentration on the assay response. The response of several ligand binding assays for total 25(OH)D was shown to be impacted by the presence of 24,25(OH)
2
D
3
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3
[24,25(OH)
2
D
3
]. These 24,25(OH)
2
D
3
results, in conjunction with concentration values assigned using RMPs for 25-hydroxyvitamin D
2
[25(OH)D
2
] and 25-hydroxyvitamin D
3
[25(OH)D
3
], provide a valuable resource for assessing the accuracy of measurements for 24,25(OH)
2
D
3
and for investigating the relationship between 24,25(OH)
2
D
3
and 25(OH)D
3
. Results for 24,25(OH)
2
D
3
using the RMP were compared to DEQAS consensus values demonstrating that the consensus values were not sufficient to assess the accuracy of measurements among different laboratories and methods. A multivariable regression analysis approach using historical DEQAS consensus values for various total 25(OH)D assays was used to assess the contribution of 24,25(OH)
2
D
3
concentration on the assay response. The response of several ligand binding assays for total 25(OH)D was shown to be impacted by the presence of 24,25(OH)
2
D
3
.</abstract><pmid>37169270</pmid><doi>10.1016/j.jsbmb.2023.106318</doi></addata></record> |
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source | Elsevier ScienceDirect Journals Complete |
title | Determination of 24,25-Dihydroxyvitamin D3 [24,25(OH)2D3] in Archived Vitamin D External Quality Assessment Scheme (DEQAS) Samples using a Reference Measurement Procedure with Comparison to DEQAS Participant Results and Assessment of Contribution of 24,25(OH)2D3 to DEQAS Assay Results |
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