CRISPR/Cas9-Mediated Knockout of tnfaip1 in Zebrafish Plays a Role in Early Development
TNF α-induced protein 1 ( ) was first identified in human umbilical vein endothelial cells and can be induced by tumor necrosis factor α (TNFα). Early studies have found that is involved in the development of many tumors and is closely associated with the neurological disorder Alzheimer's disea...
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Veröffentlicht in: | Genes 2023-04, Vol.14 (5), p.1005 |
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Hauptverfasser: | , , , , , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | TNF α-induced protein 1 (
) was first identified in human umbilical vein endothelial cells and can be induced by tumor necrosis factor α (TNFα). Early studies have found that
is involved in the development of many tumors and is closely associated with the neurological disorder Alzheimer's disease. However, little is known about the expression pattern of
under physiological conditions and its function during embryonic development. In this study, we used zebrafish as a model to illustrate the early developmental expression pattern of
and its role in early development. First, we examined the expression pattern of
during early zebrafish development using quantitative real-time PCR and whole mount in situ hybridization and found that
was highly expressed in early embryonic development and, subsequently, expression became localized to anterior embryonic structures. To investigate the function of
during early development, we constructed a model of a stably inherited
mutant using the CRISPR/Cas9 system.
mutant embryos showed significant developmental delays as well as microcephaly and microphthalmia. At the same time, we found decreased expression of the neuronal marker genes
,
, and
in
mutants. Analysis of transcriptome sequencing data revealed altered expression of the embryonic development related genes
,
,
,
,
,
,
,
,
, and
in the
mutants. These findings suggest an important role for
in the early development of zebrafish. |
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ISSN: | 2073-4425 2073-4425 |
DOI: | 10.3390/genes14051005 |