Astrocyte-derived exosomal lncRNA 4933431K23Rik modulates microglial phenotype and improves post-traumatic recovery via SMAD7 regulation

Astrocyte-microglial interaction plays a crucial role in brain injury-associated neuroinflammation. Our previous data illustrated that astrocytes secrete microRNA, leading to anti-inflammatory effects on microglia. Long non-coding RNAs participate in neuroinflammation regulation after traumatic brai...

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Veröffentlicht in:Molecular therapy 2023-05, Vol.31 (5), p.1313-1331
Hauptverfasser: He, Xuejun, Huang, Yimin, Liu, Yuan, Zhang, Xincheng, Wang, Quanji, Liu, Yanchao, Ma, Xiaopeng, Long, Xiaobing, Ruan, Yang, Lei, Hongxia, Gan, Chao, Wang, Xiaochuan, Zou, Xin, Xiong, Bo, Shu, Kai, Lei, Ting, Zhang, Huaqiu
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Sprache:eng
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Zusammenfassung:Astrocyte-microglial interaction plays a crucial role in brain injury-associated neuroinflammation. Our previous data illustrated that astrocytes secrete microRNA, leading to anti-inflammatory effects on microglia. Long non-coding RNAs participate in neuroinflammation regulation after traumatic brain injury. However, the effect of astrocytes on microglial phenotype via long non-coding RNAs and the underlying molecular mechanisms remain elusive. We used long non-coding RNA sequencing on murine astrocytes and found that exosomal long non-coding RNA 4933431K23Rik attenuated traumatic brain injury-induced microglial activation in vitro and in vivo and ameliorated cognitive function deficiency. Furthermore, microRNA and messenger RNA sequencing together with binding prediction illustrated that exosomal long non-coding RNA 4933431K23Rik up-regulates E2F7 and TFAP2C expression by sponging miR-10a-5p. Additionally, E2F7 and TFAP2C, as transcription factors, regulated microglial Smad7 expression. Using Cx3cr1-Smad7 overexpression of adeno-associated virus, microglia specifically overexpressed Smad7 in the attenuation of neuroinflammation, resulting in less cognitive deficiency after traumatic brain injury. Mechanically, overexpressed Smad7 physically binds to IκBα and inhibits its ubiquitination, preventing NF-κB signaling activation. The Smad7 activator asiaticoside alleviates neuroinflammation and protects neuronal function in traumatic brain injury mice. This study revealed that an exosomal long non-coding RNA from astrocytes attenuates microglial activation after traumatic brain injury by up-regulating Smad7, providing a potential therapeutic target. [Display omitted] Astrocyte interacts with microglia in the traumatic brain injury (TBI) conditions. In the present study, Huaqiu Zhang and colleagues revealed that astrocyte can release exosomes carrying lncRNA 4933431K23Rik that affect SMAD7 expression in microglia, resulting in attenuation of microglial pro-inflammatory phenotype switch and eventually promoting post-TBI recovery.
ISSN:1525-0016
1525-0024
DOI:10.1016/j.ymthe.2023.01.031