Gene Expression of CRAL_TRIO Family Proteins modulated by Vitamin E Deficiency in Zebrafish (Danio Rerio)
•Alpha-tocopherol transfer protein (α-TTP) is a highly conserved protein•α-TTP is a member of CRAL_TRIO, a lipid binding protein family•Vitamin E deficiency causes lipid peroxidation•Gene expression responses show impacts to phospholipid and choline metabolism An evaluation of the impact of vitamin...
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| Veröffentlicht in: | The Journal of nutritional biochemistry 2021-11, Vol.97, p.108801-108801, Article 108801 |
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| container_title | The Journal of nutritional biochemistry |
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| creator | Watt, Alexander T. Head, Brian Leonard, Scott W. Tanguay, Robyn L. Traber, Maret G. |
| description | •Alpha-tocopherol transfer protein (α-TTP) is a highly conserved protein•α-TTP is a member of CRAL_TRIO, a lipid binding protein family•Vitamin E deficiency causes lipid peroxidation•Gene expression responses show impacts to phospholipid and choline metabolism
An evaluation of the impact of vitamin E deficiency on expression of the alpha-tocopherol transfer protein (α-TTP) and related CRAL_TRIO genes was undertaken using livers from adult zebrafish based on the hypothesis that increased lipid peroxidation would modulate gene expression. Zebrafish were fed either a vitamin E sufficient (E+) or deficient (E–) diet for 9 months, then fish were euthanized, and livers were harvested. Livers from the E+ relative to E– fish contained 40-times more α-tocopherol (P |
| doi_str_mv | 10.1016/j.jnutbio.2021.108801 |
| format | Article |
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An evaluation of the impact of vitamin E deficiency on expression of the alpha-tocopherol transfer protein (α-TTP) and related CRAL_TRIO genes was undertaken using livers from adult zebrafish based on the hypothesis that increased lipid peroxidation would modulate gene expression. Zebrafish were fed either a vitamin E sufficient (E+) or deficient (E–) diet for 9 months, then fish were euthanized, and livers were harvested. Livers from the E+ relative to E– fish contained 40-times more α-tocopherol (P <0.0001) and one fourth the malondialdehyde (P = 0.0153). RNA was extracted from E+ and E– livers, then subject to evaluation of gene expression of ttpa and other genes of the CRAL_TRIO family, genes of antioxidant markers, and genes related to lipid metabolism. Ttpa expression was not altered by vitamin E status. However, one member of the CRAL_TRIO family, tyrosine-protein phosphatase non-receptor type 9 gene (ptpn9a), showed a 2.4-fold increase (P = 0.029) in E– relative to E+ livers. Further, we identified that the gene for choline kinase alpha (chka) showed a 3.0-fold increase (P = 0.010) in E– livers. These outcomes are consistent with our previous findings that show vitamin E deficiency increased lipid peroxidation causing increases in phospholipid turnover.
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An evaluation of the impact of vitamin E deficiency on expression of the alpha-tocopherol transfer protein (α-TTP) and related CRAL_TRIO genes was undertaken using livers from adult zebrafish based on the hypothesis that increased lipid peroxidation would modulate gene expression. Zebrafish were fed either a vitamin E sufficient (E+) or deficient (E–) diet for 9 months, then fish were euthanized, and livers were harvested. Livers from the E+ relative to E– fish contained 40-times more α-tocopherol (P <0.0001) and one fourth the malondialdehyde (P = 0.0153). RNA was extracted from E+ and E– livers, then subject to evaluation of gene expression of ttpa and other genes of the CRAL_TRIO family, genes of antioxidant markers, and genes related to lipid metabolism. Ttpa expression was not altered by vitamin E status. However, one member of the CRAL_TRIO family, tyrosine-protein phosphatase non-receptor type 9 gene (ptpn9a), showed a 2.4-fold increase (P = 0.029) in E– relative to E+ livers. Further, we identified that the gene for choline kinase alpha (chka) showed a 3.0-fold increase (P = 0.010) in E– livers. These outcomes are consistent with our previous findings that show vitamin E deficiency increased lipid peroxidation causing increases in phospholipid turnover.
[Display omitted]</description><subject>alpha-Tocopherol - metabolism</subject><subject>Alpha-tocopherol transfer protein (α-TTP)</subject><subject>Animals</subject><subject>Antioxidants</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - metabolism</subject><subject>Choline</subject><subject>Choline Kinase - genetics</subject><subject>Choline Kinase - metabolism</subject><subject>CRAL_TRIO</subject><subject>Gene Expression</subject><subject>Lipid Metabolism - genetics</subject><subject>Liver - metabolism</subject><subject>Malondialdehyde - metabolism</subject><subject>Protein Tyrosine Phosphatases, Non-Receptor - genetics</subject><subject>Protein Tyrosine Phosphatases, Non-Receptor - metabolism</subject><subject>Ptpn9a</subject><subject>SEC14</subject><subject>Vitamin E Deficiency - genetics</subject><subject>Vitamin E Deficiency - metabolism</subject><subject>Zebrafish</subject><subject>Zebrafish Proteins - genetics</subject><subject>Zebrafish Proteins - metabolism</subject><issn>0955-2863</issn><issn>1873-4847</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1vEzEQhi0EoqHwE0A-0sMGe_2xzglVaVoqRSqKCgcultce04kSO7I3Ffn3bEkpcOI0mo_3nZmHkLecTTnj-sN6uk77occ8bVnLx5oxjD8jE2460Ugju-dkwmZKNa3R4oS8qnXNGGul0i_JiZCcz7RgE4JXkIAufuwK1Io50RzpfHW-tLer6xt66ba4OdDPJQ-AqdJtDvuNGyDQ_kC_4jC2E13QC4joEZI_0DH_Bn1xEesdfX_hEma6goL57DV5Ed2mwpvHeEq-XC5u55-a5c3V9fx82XipzdBwoTjTTEIbGUAMvfJcqi6GTnfMzAxT41PSSaek70wXdeDGtwJ8kLI3QopT8vHou9v3Wwge0lDcxu4Kbl052OzQ_ttJeGe_53s7Ym1nTHSjgzo6-JJrLRCfxJw9TGm7to_w7QN8e4Q_6t79vflJ9Zv2n9Ng_P8eodj6CxsELOAHGzL-Z8VPsvaYnA</recordid><startdate>20211101</startdate><enddate>20211101</enddate><creator>Watt, Alexander T.</creator><creator>Head, Brian</creator><creator>Leonard, Scott W.</creator><creator>Tanguay, Robyn L.</creator><creator>Traber, Maret G.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-7520-2227</orcidid><orcidid>https://orcid.org/0000-0002-2892-4024</orcidid></search><sort><creationdate>20211101</creationdate><title>Gene Expression of CRAL_TRIO Family Proteins modulated by Vitamin E Deficiency in Zebrafish (Danio Rerio)</title><author>Watt, Alexander T. ; Head, Brian ; Leonard, Scott W. ; Tanguay, Robyn L. ; Traber, Maret G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c468t-13510604e2f0eefdb5c1457fd7670898059554a4a54c787f6d18c23ecd44b8343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>alpha-Tocopherol - metabolism</topic><topic>Alpha-tocopherol transfer protein (α-TTP)</topic><topic>Animals</topic><topic>Antioxidants</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - metabolism</topic><topic>Choline</topic><topic>Choline Kinase - genetics</topic><topic>Choline Kinase - metabolism</topic><topic>CRAL_TRIO</topic><topic>Gene Expression</topic><topic>Lipid Metabolism - genetics</topic><topic>Liver - metabolism</topic><topic>Malondialdehyde - metabolism</topic><topic>Protein Tyrosine Phosphatases, Non-Receptor - genetics</topic><topic>Protein Tyrosine Phosphatases, Non-Receptor - metabolism</topic><topic>Ptpn9a</topic><topic>SEC14</topic><topic>Vitamin E Deficiency - genetics</topic><topic>Vitamin E Deficiency - metabolism</topic><topic>Zebrafish</topic><topic>Zebrafish Proteins - genetics</topic><topic>Zebrafish Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Watt, Alexander T.</creatorcontrib><creatorcontrib>Head, Brian</creatorcontrib><creatorcontrib>Leonard, Scott W.</creatorcontrib><creatorcontrib>Tanguay, Robyn L.</creatorcontrib><creatorcontrib>Traber, Maret G.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of nutritional biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Watt, Alexander T.</au><au>Head, Brian</au><au>Leonard, Scott W.</au><au>Tanguay, Robyn L.</au><au>Traber, Maret G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gene Expression of CRAL_TRIO Family Proteins modulated by Vitamin E Deficiency in Zebrafish (Danio Rerio)</atitle><jtitle>The Journal of nutritional biochemistry</jtitle><addtitle>J Nutr Biochem</addtitle><date>2021-11-01</date><risdate>2021</risdate><volume>97</volume><spage>108801</spage><epage>108801</epage><pages>108801-108801</pages><artnum>108801</artnum><issn>0955-2863</issn><eissn>1873-4847</eissn><abstract>•Alpha-tocopherol transfer protein (α-TTP) is a highly conserved protein•α-TTP is a member of CRAL_TRIO, a lipid binding protein family•Vitamin E deficiency causes lipid peroxidation•Gene expression responses show impacts to phospholipid and choline metabolism
An evaluation of the impact of vitamin E deficiency on expression of the alpha-tocopherol transfer protein (α-TTP) and related CRAL_TRIO genes was undertaken using livers from adult zebrafish based on the hypothesis that increased lipid peroxidation would modulate gene expression. Zebrafish were fed either a vitamin E sufficient (E+) or deficient (E–) diet for 9 months, then fish were euthanized, and livers were harvested. Livers from the E+ relative to E– fish contained 40-times more α-tocopherol (P <0.0001) and one fourth the malondialdehyde (P = 0.0153). RNA was extracted from E+ and E– livers, then subject to evaluation of gene expression of ttpa and other genes of the CRAL_TRIO family, genes of antioxidant markers, and genes related to lipid metabolism. Ttpa expression was not altered by vitamin E status. However, one member of the CRAL_TRIO family, tyrosine-protein phosphatase non-receptor type 9 gene (ptpn9a), showed a 2.4-fold increase (P = 0.029) in E– relative to E+ livers. Further, we identified that the gene for choline kinase alpha (chka) showed a 3.0-fold increase (P = 0.010) in E– livers. These outcomes are consistent with our previous findings that show vitamin E deficiency increased lipid peroxidation causing increases in phospholipid turnover.
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| subjects | alpha-Tocopherol - metabolism Alpha-tocopherol transfer protein (α-TTP) Animals Antioxidants Carrier Proteins - genetics Carrier Proteins - metabolism Choline Choline Kinase - genetics Choline Kinase - metabolism CRAL_TRIO Gene Expression Lipid Metabolism - genetics Liver - metabolism Malondialdehyde - metabolism Protein Tyrosine Phosphatases, Non-Receptor - genetics Protein Tyrosine Phosphatases, Non-Receptor - metabolism Ptpn9a SEC14 Vitamin E Deficiency - genetics Vitamin E Deficiency - metabolism Zebrafish Zebrafish Proteins - genetics Zebrafish Proteins - metabolism |
| title | Gene Expression of CRAL_TRIO Family Proteins modulated by Vitamin E Deficiency in Zebrafish (Danio Rerio) |
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