Gene Expression of CRAL_TRIO Family Proteins modulated by Vitamin E Deficiency in Zebrafish (Danio Rerio)

•Alpha-tocopherol transfer protein (α-TTP) is a highly conserved protein•α-TTP is a member of CRAL_TRIO, a lipid binding protein family•Vitamin E deficiency causes lipid peroxidation•Gene expression responses show impacts to phospholipid and choline metabolism An evaluation of the impact of vitamin E deficiency on expression of the alpha-tocopherol transfer protein (α-TTP) and related CRAL_TRIO genes was undertaken using livers from adult zebrafish based on the hypothesis that increased lipid peroxidation would modulate gene expression. Zebrafish were fed either a vitamin E sufficient (E+) or deficient (E–) diet for 9 months, then fish were euthanized, and livers were harvested. Livers from the E+ relative to E– fish contained 40-times more α-tocopherol (P