Single step method for high yield human platelet lysate production

Background We aimed to develop a single step method for the production of human platelet lysate (hPL). The method must result in high hPL yields, be closed system and avoid heparin use. Study Design and Methods The method aimed at using glass beads and calcium. An optimal concentration of calcium and glass beads was determined by serial dilution. This was translated to a novel method and compared to known methods: freeze‐thawing and high calcium. Quality outcome measures were transmittance, fibrinogen and growth factor content, and cell doubling time. Results An optimal concentration of 5 mM Ca2+ and 0.2 g/ml glass beads resulted in hPL with yields of 92% ± 1% (n = 50) independent of source material (apheresis or buffy coat‐derived). The transmittance was highest (56% ± 9%) compared to known methods (