Cycloaddition enabled mutational profiling of 5-vinyluridine in RNA
We report the detection of 5-vinyluridine (5-VUrd) in RNA at single nucleotide resolution via mutational profiling. Maleimide cycloadducts with 5-VUrd in RNA cause a stop in primer extension during reverse transcription, and the full-length cDNA product from reverse transcription contains misincorpo...
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| Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2023-03, Vol.59 (22), p.3257-326 |
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| container_title | Chemical communications (Cambridge, England) |
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| creator | Gupta, Mrityunjay Wang, Jingtian Garfio, Chely M Vandewalle, Abigail Spitale, Robert C |
| description | We report the detection of 5-vinyluridine (5-VUrd) in RNA at single nucleotide resolution
via
mutational profiling. Maleimide cycloadducts with 5-VUrd in RNA cause a stop in primer extension during reverse transcription, and the full-length cDNA product from reverse transcription contains misincorporation across the cycloadduct site.
We report the detection of 5-vinyluridine (5-VUrd) in RNA at single nucleotide resolution
via
mutational profiling. |
| doi_str_mv | 10.1039/d3cc00043e |
| format | Article |
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via
mutational profiling. Maleimide cycloadducts with 5-VUrd in RNA cause a stop in primer extension during reverse transcription, and the full-length cDNA product from reverse transcription contains misincorporation across the cycloadduct site.
We report the detection of 5-vinyluridine (5-VUrd) in RNA at single nucleotide resolution
via
mutational profiling.</description><identifier>ISSN: 1359-7345</identifier><identifier>EISSN: 1364-548X</identifier><identifier>DOI: 10.1039/d3cc00043e</identifier><identifier>PMID: 36815680</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Cycloaddition ; Cycloaddition Reaction ; Nucleotides ; RNA - genetics</subject><ispartof>Chemical communications (Cambridge, England), 2023-03, Vol.59 (22), p.3257-326</ispartof><rights>Copyright Royal Society of Chemistry 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c429t-dd76bbce7067e0cc2c92fe9651ab5f54b28a565262bb66a3dd3f74444c84de763</citedby><cites>FETCH-LOGICAL-c429t-dd76bbce7067e0cc2c92fe9651ab5f54b28a565262bb66a3dd3f74444c84de763</cites><orcidid>0000-0002-9118-1681 ; 0000-0002-3511-8098</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36815680$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gupta, Mrityunjay</creatorcontrib><creatorcontrib>Wang, Jingtian</creatorcontrib><creatorcontrib>Garfio, Chely M</creatorcontrib><creatorcontrib>Vandewalle, Abigail</creatorcontrib><creatorcontrib>Spitale, Robert C</creatorcontrib><title>Cycloaddition enabled mutational profiling of 5-vinyluridine in RNA</title><title>Chemical communications (Cambridge, England)</title><addtitle>Chem Commun (Camb)</addtitle><description>We report the detection of 5-vinyluridine (5-VUrd) in RNA at single nucleotide resolution
via
mutational profiling. Maleimide cycloadducts with 5-VUrd in RNA cause a stop in primer extension during reverse transcription, and the full-length cDNA product from reverse transcription contains misincorporation across the cycloadduct site.
We report the detection of 5-vinyluridine (5-VUrd) in RNA at single nucleotide resolution
via
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via
mutational profiling. Maleimide cycloadducts with 5-VUrd in RNA cause a stop in primer extension during reverse transcription, and the full-length cDNA product from reverse transcription contains misincorporation across the cycloadduct site.
We report the detection of 5-vinyluridine (5-VUrd) in RNA at single nucleotide resolution
via
mutational profiling.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>36815680</pmid><doi>10.1039/d3cc00043e</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0002-9118-1681</orcidid><orcidid>https://orcid.org/0000-0002-3511-8098</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1359-7345 |
| ispartof | Chemical communications (Cambridge, England), 2023-03, Vol.59 (22), p.3257-326 |
| issn | 1359-7345 1364-548X |
| language | eng |
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| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Cycloaddition Cycloaddition Reaction Nucleotides RNA - genetics |
| title | Cycloaddition enabled mutational profiling of 5-vinyluridine in RNA |
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