Cycloaddition enabled mutational profiling of 5-vinyluridine in RNA

Cycloaddition enabled mutational profiling of 5-vinyluridine in RNA

We report the detection of 5-vinyluridine (5-VUrd) in RNA at single nucleotide resolution via mutational profiling. Maleimide cycloadducts with 5-VUrd in RNA cause a stop in primer extension during reverse transcription, and the full-length cDNA product from reverse transcription contains misincorpo...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2023-03, Vol.59 (22), p.3257-326
Hauptverfasser: Gupta, Mrityunjay, Wang, Jingtian, Garfio, Chely M, Vandewalle, Abigail, Spitale, Robert C
Format: Artikel
Sprache:eng
Schlagworte:
Cycloaddition
Cycloaddition Reaction
Nucleotides
RNA - genetics
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Zusammenfassung:We report the detection of 5-vinyluridine (5-VUrd) in RNA at single nucleotide resolution via mutational profiling. Maleimide cycloadducts with 5-VUrd in RNA cause a stop in primer extension during reverse transcription, and the full-length cDNA product from reverse transcription contains misincorporation across the cycloadduct site. We report the detection of 5-vinyluridine (5-VUrd) in RNA at single nucleotide resolution via mutational profiling.
ISSN:1359-7345
1364-548X
DOI:10.1039/d3cc00043e