Effect of different application strategies of universal adhesive used for immediate and delayed dentin sealing on the microtensile bond strength of self-adhesive resin cement to dentin with and without aging

Bond strength of indirect restorations is a very important issue that should be given special attention. Immediate dentin sealing (IDS) technique has been suggested in recent years. The aim of the present study was to investigate the effect of different strategies of universal adhesive application for immediate and delayed dentin sealing (DDS) on the microtensile bond strength (μTBS) of self-adhesive resin cement with and without aging. In this experimental study, 24 healthy human third molars were selected. After exposing the occlusal dentin, the teeth were randomly divided into two groups of 12 based on the All-Bond Universal adhesive application strategy (etch-and-rinse or self-etch). Each group was further subdivided into two (n=6) based on IDS or DDS technique. Then composite blocks were cemented on the occlusal surface with self-adhesive resin cement. After cutting the samples into 1 mm2 cross-sections, half of the samples of each subgroup were subjected to µTBS test after one week, and the other half were tested under μTBS after 10,000 thermal cycles. Data were analyzed using three-way ANOVA (