Interferon-γ Receptor β-Chain Expression and Formation of α- and β-Chain Complexes after Receptor Conjugation on Human Peripheral Eosinophils

Background: It has been unclear whether or not the interferon-γ receptor (IFN-γR) on human peripheral eosinophils is completely functional. Accordingly, we examined the expression of IFN-γR α- and β-chains and the association of the two chains after binding of IFN-γ to the receptor. Methods: Periphe...

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Veröffentlicht in:International archives of allergy and immunology 1998-01, Vol.117 (Suppl 1), p.72-76
Hauptverfasser: Ishiharu, Chie, Ochiai, Kenichi, Kagami, Masaru, Matsuyama, Gou, Koya, Niroku, Tomioka, Hisao
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Sprache:eng
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Zusammenfassung:Background: It has been unclear whether or not the interferon-γ receptor (IFN-γR) on human peripheral eosinophils is completely functional. Accordingly, we examined the expression of IFN-γR α- and β-chains and the association of the two chains after binding of IFN-γ to the receptor. Methods: Peripheral blood eosinophils were obtained from 8 patients (bronchial asthma, n=6, and hypereosinophilic syndrome, n=2), and expression of the α- and β-chain was investigated by flow cytometry with specific antibodies. Expression of mRNA for the α- and β-subunits was also investigated by the reverse transcriptase-polymerase chain reaction. Results: Eosinophils from all the patients were positive for both the α- and β-chains by flow cytometry. In addition, mRNA for both chains was detected by the polymerase chain reaction. Furthermore, coprecipitation of the α- and β-chains was only found after eosinophil stimulation with IFN-γ. Conclusions: Human peripheral eosinophils apparently express both the α- and β-chains of the IFN-γR, and the two chains may only form the receptor complex after ligand binding.
ISSN:1018-2438
1423-0097
DOI:10.1159/000063280