Construction of specific DNA probe for the detection of Salmonella in food

The Salmonella specific DNA fragment from genomic DNA of S. typhimurium ATCC 23566 was cloned in E. coli and successfully used as a digoxigenin labeled probe for detecting the presence of Salmonella serotypes in both artificially contaminated food and natural contaminated food samples.

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Bibliographische Detailangaben
Veröffentlicht in:	Southeast Asian journal of tropical medicine and public health 1997-03, Vol.28 (1), p.73-81
Hauptverfasser:	PILANTANAPAK, A, JAYANETRA, P, PANBANGRED, W, KLUNGTHONG, C, BANGTRAKULNONTH, A
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial diseases Bacterial diseases of the digestive system and abdomen Biological and medical sciences Cloning, Molecular Colony Count, Microbial Digoxigenin DNA Probes - genetics DNA, Bacterial - genetics Escherichia coli - genetics Food Microbiology Human bacterial diseases Humans Immunoblotting Infectious diseases Medical sciences Salmonella Food Poisoning - microbiology Salmonella Food Poisoning - prevention & control Salmonella typhimurium - genetics Tropical medicine
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Beschreibung
Zusammenfassung:	The Salmonella specific DNA fragment from genomic DNA of S. typhimurium ATCC 23566 was cloned in E. coli and successfully used as a digoxigenin labeled probe for detecting the presence of Salmonella serotypes in both artificially contaminated food and natural contaminated food samples.
ISSN:	0125-1562