DNA Sequence and Nucleosome Placement on the Murine Fibroblast Growth Factor-4 Gene

A large portion of the 5′ flanking region of the fibroblast growth factor-4 (PGF-4) gene has been sequenced, but only 38 bp of sequence information past the end of the last exon of this gene has been described. In this study, a total of 2769 bp of nucleotide sequence downstream of the last exon of F...

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Veröffentlicht in:	DNA sequence 1997, Vol.7 (2), p.117-121
Hauptverfasser:	Wilder, Phillip J., Mountjoy, Charles, Macleod, Michael C., Rizzino, Angie
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals B1 repetitive element Base Sequence Binding Sites - genetics Exons Fibroblast Growth Factor 4 fibroblast growth factor 4 (FGF-4) Fibroblast Growth Factors - genetics Genes - genetics Mice Molecular Sequence Data nucleosome positioning Nucleosomes - chemistry Nucleosomes - genetics Proto-Oncogene Proteins - genetics Sequence Analysis, DNA snJRNA U1
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Zusammenfassung:	A large portion of the 5′ flanking region of the fibroblast growth factor-4 (PGF-4) gene has been sequenced, but only 38 bp of sequence information past the end of the last exon of this gene has been described. In this study, a total of 2769 bp of nucleotide sequence downstream of the last exon of FGF-4 (GenBank #U43515) was determined by a combination of deletional subcloning and primer walking. In addition, we have used the theoretical algorithm of Calladine and Drew to predict the rotational positioning of nucleosomes throughout the entire FGF-4 gene.
ISSN:	1042-5179 1029-2365
DOI:	10.3109/10425179709020158