Effects of neutralizing antibodies on cytokine treatment for anti-GBM nephritis in mouse

The process of glomerular injury in nephrotoxic serum nephritis (NTN) is dependent on proinflammatory cytokines. In the present investigation, we assessed the actions of neutralizing antibody against IL-1, 9, TNF-α, IL-6 and TGF-β1 on glomerular injury. Marked increase in IL-1 and IL-6 was detected in cultured glomeruli of NTN in mice throughout the experiments from disease induction. Protein of TNF-α and TGF-$1 also increased in NTN mice 1 day after disease induction. Treatment with either IL-l, S or TNF- a neutralizing antibody reduced proteinuria from 71 ± 11.2 m g/24 hr to 32.2±6.0 (P