p-maleimidophenyl isocyanate: a novel heterobifunctional linker for hydroxyl to thiol coupling

p-Maleimidophenyl isocyanate (PMPI, 1) is a heterobifunctional cross-linking agent useful for thiol to hydroxyl coupling. Several maleimide-activated compounds were prepared and characterized and then shown to be reactive with thiol-containing proteins. Examples include activation of vitamin B12, di...

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Veröffentlicht in:	Bioconjugate chemistry 1993-05, Vol.4 (3), p.212
Hauptverfasser:	Annunziato, M E, Patel, U S, Ranade, M, Palumbo, P S
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkaline Phosphatase - chemistry Amino Acid Sequence Cross-Linking Reagents - chemical synthesis Cross-Linking Reagents - chemistry Cyanates - chemical synthesis Cyanates - chemistry Digoxigenin - chemistry Drug Stability Estradiol - chemistry Haptens - chemistry Maleimides - chemical synthesis Maleimides - chemistry Molecular Sequence Data Peptides - chemistry Sulfhydryl Compounds - chemistry Vitamin B 12 - chemistry
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Beschreibung
Zusammenfassung:	p-Maleimidophenyl isocyanate (PMPI, 1) is a heterobifunctional cross-linking agent useful for thiol to hydroxyl coupling. Several maleimide-activated compounds were prepared and characterized and then shown to be reactive with thiol-containing proteins. Examples include activation of vitamin B12, digoxigenin, digitoxigenin, estradiol, progesterone, and some serine-containing peptides.
ISSN:	1043-1802
DOI:	10.1021/bc00021a005