CYTOSTATIC ACTION OF HUMAN FIBROBLAST INTERFERON IN DIRECT GROWTH INHIBITION: EFFECT ON HUMAN MALIGNANT MELANOMA
The mode of action of the direct antiproliferative effect of human fibroblast interferon (HuIFN-β) on tumor cells was analyzed in vitro with a human malignant melanoma cell line, HMV-1. HuIFN-β inhibited the growth of HMV-1 cells in a time-dependent fashion (50%-inhibition concentration:
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| Veröffentlicht in: | GANN Japanese Journal of Cancer Research 1983, Vol.74(3), pp.452-458 |
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| container_end_page | 458 |
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| container_issue | 3 |
| container_start_page | 452 |
| container_title | GANN Japanese Journal of Cancer Research |
| container_volume | 74 |
| creator | NAGAO, Shin'ichi TOHGO, Akiko KOHNO, Morihiro OGAWA, Hidemasa |
| description | The mode of action of the direct antiproliferative effect of human fibroblast interferon (HuIFN-β) on tumor cells was analyzed in vitro with a human malignant melanoma cell line, HMV-1. HuIFN-β inhibited the growth of HMV-1 cells in a time-dependent fashion (50%-inhibition concentration: |
| doi_str_mv | 10.20772/cancersci1959.74.3_452 |
| format | Article |
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HuIFN-β inhibited the growth of HMV-1 cells in a time-dependent fashion (50%-inhibition concentration: <50IU/ml). Its action was cytostatic. DNA synthesis was inhibited before the antitumor effect appeared, and apparent dose-dependent inhibitions of RNA and protein synthesis were induced. An increase of cell protein content was seen with the occurrence of growth inhibition. Increase of cell volume and prolongation of doubling time were seen from the second day after the addition, which corresponded well with the effects of HuIFN-β on the cell cycle (the accumulation of cells in the S phase). These results indicate that the direct antitumor effect of HuIFN-β on HMV-1 cells may result from cell cycle retardation mediated through obstruction of DNA synthesis.</description><identifier>ISSN: 0016-450X</identifier><identifier>DOI: 10.20772/cancersci1959.74.3_452</identifier><identifier>PMID: 6884703</identifier><language>eng</language><publisher>Tokyo: The Japanese Cancer Association</publisher><subject>Antineoplastic agents ; Biological and medical sciences ; Cell Cycle - drug effects ; Cell Line ; Chemotherapy ; DNA, Neoplasm - biosynthesis ; Human fibroblast interferon (HuIFN-β) ; Human malignant melanoma ; Humans ; Interferon Type I - therapeutic use ; Medical sciences ; Melanoma - metabolism ; Melanoma - therapy ; Mode of action ; Neoplasm Proteins - biosynthesis ; Pharmacology. Drug treatments ; RNA, Neoplasm - biosynthesis</subject><ispartof>GANN Japanese Journal of Cancer Research, 1983, Vol.74(3), pp.452-458</ispartof><rights>The Japanese Cancer Association</rights><rights>1984 INIST-CNRS</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9406177$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6884703$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>NAGAO, Shin'ichi</creatorcontrib><creatorcontrib>TOHGO, Akiko</creatorcontrib><creatorcontrib>KOHNO, Morihiro</creatorcontrib><creatorcontrib>OGAWA, Hidemasa</creatorcontrib><title>CYTOSTATIC ACTION OF HUMAN FIBROBLAST INTERFERON IN DIRECT GROWTH INHIBITION: EFFECT ON HUMAN MALIGNANT MELANOMA</title><title>GANN Japanese Journal of Cancer Research</title><addtitle>GANN Japanese Journal of Cancer Research</addtitle><description>The mode of action of the direct antiproliferative effect of human fibroblast interferon (HuIFN-β) on tumor cells was analyzed in vitro with a human malignant melanoma cell line, HMV-1. HuIFN-β inhibited the growth of HMV-1 cells in a time-dependent fashion (50%-inhibition concentration: <50IU/ml). Its action was cytostatic. DNA synthesis was inhibited before the antitumor effect appeared, and apparent dose-dependent inhibitions of RNA and protein synthesis were induced. An increase of cell protein content was seen with the occurrence of growth inhibition. Increase of cell volume and prolongation of doubling time were seen from the second day after the addition, which corresponded well with the effects of HuIFN-β on the cell cycle (the accumulation of cells in the S phase). These results indicate that the direct antitumor effect of HuIFN-β on HMV-1 cells may result from cell cycle retardation mediated through obstruction of DNA synthesis.</description><subject>Antineoplastic agents</subject><subject>Biological and medical sciences</subject><subject>Cell Cycle - drug effects</subject><subject>Cell Line</subject><subject>Chemotherapy</subject><subject>DNA, Neoplasm - biosynthesis</subject><subject>Human fibroblast interferon (HuIFN-β)</subject><subject>Human malignant melanoma</subject><subject>Humans</subject><subject>Interferon Type I - therapeutic use</subject><subject>Medical sciences</subject><subject>Melanoma - metabolism</subject><subject>Melanoma - therapy</subject><subject>Mode of action</subject><subject>Neoplasm Proteins - biosynthesis</subject><subject>Pharmacology. Drug treatments</subject><subject>RNA, Neoplasm - biosynthesis</subject><issn>0016-450X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkUFPwjAUgHvQIEF-grEHr2C7du3mrcyNNRlbMkrU09JuRUeAkA0P_ntLIBjfoS9539eX11cAHjGaeohz77nW-9p2fd3i0A-nnE5JRX3vBgwRwmxCffR-B8Z9v0EumMc9zgZgwIKAckSG4BB9qGKphJIRFJGSRQ6LBKarhchhImdlMcvEUkGZq7hM4tJhmcNXWcaRgvOyeFOpK6RyJk9XX2CcJCfitHOLhcjkPBe5gos4E3mxEPfgdq23vR1f8gisklhF6SQr5jIS2WRDcXCcGM0DGxLjUV-bgDDmo9AEzM2MkCY6NIxi3gTYo2uCuW8x8ZGtDefcb4xpAjICD-e-h2-zs0116Nqd7n6qy8sdf7pw3dd6u-7cGtv-qoUUMcy50-RZ2_RH_WmvXHfHtt7a6t_2K04rcj7cF_w5X7qr7J78ArmDeYQ</recordid><startdate>19830101</startdate><enddate>19830101</enddate><creator>NAGAO, Shin'ichi</creator><creator>TOHGO, Akiko</creator><creator>KOHNO, Morihiro</creator><creator>OGAWA, Hidemasa</creator><general>The Japanese Cancer Association</general><general>Japan Publications Trading</general><general>Japanese Cancer Association</general><general>Cancer Institute</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>19830101</creationdate><title>CYTOSTATIC ACTION OF HUMAN FIBROBLAST INTERFERON IN DIRECT GROWTH INHIBITION: EFFECT ON HUMAN MALIGNANT MELANOMA</title><author>NAGAO, Shin'ichi ; TOHGO, Akiko ; KOHNO, Morihiro ; OGAWA, Hidemasa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j418t-ba78e93b245ab8366509b8670300a3a9b6417d8124f3175e1350ecb7775dbbd83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Antineoplastic agents</topic><topic>Biological and medical sciences</topic><topic>Cell Cycle - drug effects</topic><topic>Cell Line</topic><topic>Chemotherapy</topic><topic>DNA, Neoplasm - biosynthesis</topic><topic>Human fibroblast interferon (HuIFN-β)</topic><topic>Human malignant melanoma</topic><topic>Humans</topic><topic>Interferon Type I - therapeutic use</topic><topic>Medical sciences</topic><topic>Melanoma - metabolism</topic><topic>Melanoma - therapy</topic><topic>Mode of action</topic><topic>Neoplasm Proteins - biosynthesis</topic><topic>Pharmacology. Drug treatments</topic><topic>RNA, Neoplasm - biosynthesis</topic><toplevel>online_resources</toplevel><creatorcontrib>NAGAO, Shin'ichi</creatorcontrib><creatorcontrib>TOHGO, Akiko</creatorcontrib><creatorcontrib>KOHNO, Morihiro</creatorcontrib><creatorcontrib>OGAWA, Hidemasa</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>GANN Japanese Journal of Cancer Research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>NAGAO, Shin'ichi</au><au>TOHGO, Akiko</au><au>KOHNO, Morihiro</au><au>OGAWA, Hidemasa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CYTOSTATIC ACTION OF HUMAN FIBROBLAST INTERFERON IN DIRECT GROWTH INHIBITION: EFFECT ON HUMAN MALIGNANT MELANOMA</atitle><jtitle>GANN Japanese Journal of Cancer Research</jtitle><addtitle>GANN Japanese Journal of Cancer Research</addtitle><date>1983-01-01</date><risdate>1983</risdate><volume>74</volume><issue>3</issue><spage>452</spage><epage>458</epage><pages>452-458</pages><issn>0016-450X</issn><abstract>The mode of action of the direct antiproliferative effect of human fibroblast interferon (HuIFN-β) on tumor cells was analyzed in vitro with a human malignant melanoma cell line, HMV-1. HuIFN-β inhibited the growth of HMV-1 cells in a time-dependent fashion (50%-inhibition concentration: <50IU/ml). Its action was cytostatic. DNA synthesis was inhibited before the antitumor effect appeared, and apparent dose-dependent inhibitions of RNA and protein synthesis were induced. An increase of cell protein content was seen with the occurrence of growth inhibition. Increase of cell volume and prolongation of doubling time were seen from the second day after the addition, which corresponded well with the effects of HuIFN-β on the cell cycle (the accumulation of cells in the S phase). These results indicate that the direct antitumor effect of HuIFN-β on HMV-1 cells may result from cell cycle retardation mediated through obstruction of DNA synthesis.</abstract><cop>Tokyo</cop><pub>The Japanese Cancer Association</pub><pmid>6884703</pmid><doi>10.20772/cancersci1959.74.3_452</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | GANN Japanese Journal of Cancer Research, 1983, Vol.74(3), pp.452-458 |
| issn | 0016-450X |
| language | eng |
| recordid | cdi_pubmed_primary_6884703 |
| source | J-STAGE Free; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Antineoplastic agents Biological and medical sciences Cell Cycle - drug effects Cell Line Chemotherapy DNA, Neoplasm - biosynthesis Human fibroblast interferon (HuIFN-β) Human malignant melanoma Humans Interferon Type I - therapeutic use Medical sciences Melanoma - metabolism Melanoma - therapy Mode of action Neoplasm Proteins - biosynthesis Pharmacology. Drug treatments RNA, Neoplasm - biosynthesis |
| title | CYTOSTATIC ACTION OF HUMAN FIBROBLAST INTERFERON IN DIRECT GROWTH INHIBITION: EFFECT ON HUMAN MALIGNANT MELANOMA |
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