Complete mRNA Coding Sequence of the Acetylcholine Binding α -Subunit of Torpedo marmorata Acetylcholine Receptor: A Model for the Transmembrane Organization of the Polypeptide Chain

Complete mRNA Coding Sequence of the Acetylcholine Binding α -Subunit of Torpedo marmorata Acetylcholine Receptor: A Model for the Transmembrane Organization of the Polypeptide Chain

A 1,350-base-pair-long cDNA clone, named pα -2, was isolated by hybridization to the previously characterized clone pα -1 and found to be specific for the α -subunit of the Torpedo marmorata acetylcholine receptor. The nucleotide sequences of both cDNA inserts were analyzed and the sequence of the c...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1983-04, Vol.80 (7), p.2067-2071
Hauptverfasser: Devillers-Thiery, Anne, Giraudat, Jerome, Bentaboulet, Martine, Changeux, Jean-Pierre
Format: Artikel
Sprache:eng
Schlagworte:
acetylcholine
Acetylcholine - metabolism
Amino Acid Sequence
Amino acids
Animals
Base Sequence
Binding Sites
Complementary DNA
DNA
Generally accepted auditing standards
Macromolecular Substances
Marine
Membrane Proteins - genetics
membranes
Messenger RNA
Models, Biological
mRNA
neurophysiology
nucleotide sequence
Nucleotide sequences
Nucleotides
P branes
Protein Conformation
Receptors, Cholinergic - genetics
RNA
RNA, Messenger - genetics
String theory
Synaptic Membranes - ultrastructure
Torpedo
Torpedo marmorata
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Zusammenfassung:A 1,350-base-pair-long cDNA clone, named pα -2, was isolated by hybridization to the previously characterized clone pα -1 and found to be specific for the α -subunit of the Torpedo marmorata acetylcholine receptor. The nucleotide sequences of both cDNA inserts were analyzed and the sequence of the complete coding region and part of the 5′and 3′untranslated regions of the α -chain mRNA was determined. The complete amino acid sequence of the α -chain precursor is presented and used to develop a model for the transmembrane organization of the polypeptide.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.80.7.2067