A Specific Mutation Abolishing Na+/H+Antiport Activity in Hamster Fibroblasts Precludes Growth at Neutral and Acidic pH

A H+-suicide technique based on the reversibility of Na+/H+antiport was developed for the selection of mutants deficient in this membrane-bound activity. The strategy was to use the Na+/H+antiporter as a H+-vector killing device. Chinese hamster lung fibroblasts (CCL39) were loaded with LiCl and inc...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1984-08, Vol.81 (15), p.4833-4837
Hauptverfasser:	Pouysségur, Jacques, Sardet, Claude, Franchi, Arlette, L'Allemain, Gilles, Paris, Sonia
Format:	Artikel
Sprache:	eng
Schlagworte:	Acid-Base Equilibrium Animal cells Animals Antiporters B lymphocytes Carrier Proteins - genetics Cell Cycle Cell growth Cell Line Cells Cricetinae Delta cells Hybrid Cells - metabolism Hydrogen-Ion Concentration Mutation Protons Sodium - metabolism Sodium-Hydrogen Exchangers Suicide Viability
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	A H+-suicide technique based on the reversibility of Na+/H+antiport was developed for the selection of mutants deficient in this membrane-bound activity. The strategy was to use the Na+/H+antiporter as a H+-vector killing device. Chinese hamster lung fibroblasts (CCL39) were loaded with LiCl and incubated in Na+-, Li+-free choline Cl saline solution (pH 5.5). Under these conditions, intracellular pH dropped in 5 min from 7.1 to 4.8, leading to a rapid loss of cell viability (
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.81.15.4833