Effect of Oral Administration of N,N′-2,7-Fluorenylenebisacetamide Combined With Traumatic Ulcers of the Glandular Stomach of Buffalo Rats

Male weanling rats of the Buffalo strain were castrated prior to continuous feeding of a diet containing 0.025 percent of N,N′-2,7-fluorenylenebisacctamide. In addition to the castration, one group was also subcutaneously implanted with diethylstilbestrol pellets. Traumatic ulcers of the glandular stomach were produced in the other castrated animals at intervals of 1, 3, 5, and 7 months after the carcinogenic diet was started. Only one carcinoma of the glandular stomach was induced in a castrated rat that received a traumatic ulcer 1 and 7 months after the carcinogenic diet was begun, Two adenocarcinomas developing at the gastroduodenal junction may have originated either from Brunner's glands or from duodenal mucosa. All the castrated animals treated with estrogen that survived 6 months or longer had mammary carcinomas. Incidence of liver tumors in this group was similar to that in the other castrated groups. Castrated rats subjected to traumatic ulcer 3 months after the carcinogenic diet was started had less liver cancer than the noncastrated group. However, traumatic ulceration of the stomach had no effect on the development of cancer of the glandular stomach.