Effects on Toxicity and on Enzyme Activity of the Interactions Between Aldrin, Chlordane, Piperonyl Butoxide, and Banol in Rats

Summary Rats were administered aldrin, chlordane, piperonyl butoxide, aldrin plus piperonyl butoxide, or chlordane plus piperonyl butoxide, and the toxicity and effects on some serum and liver enzyme activities after subsequent Banol administration was investigated. Aldrin and chlordane pre-treatment decreased the mortality after a single oral dose of Banol (2 X LD50) from 85 to 35%. Pretreatment with the organochlorines and piperonyl butoxide together abolished the protective action of the organochlorines alone. Piperonyl butoxide pretreatment alone had no effect on the toxicity of Banol. Serum aliesterase (AliE) and liver aniline hydroxylase and nitroreductase activities were markedly increased after organochlorine pretreatment and also after organochlorine-piperonyl butoxide pretreatment, while liver AliE was less markedly increased and increases in enzyme activity after piperonyl butoxide pretreatment were minimal. Administration of Banol to rats similarly pretreated decreased liver AliE, but not serum AliE or liver aniline hydroxylase or nitroreductase.