Studies on chloroplast development and replication in Euglena: II. Identification of two different deoxyribonucleases

Extracts of Euglena contain at least two DNases. One, referred to as “Euglena acid DNase,” has a pH optimum of 4.6, and exhibits enzymic and purification properties similar to DNase II-like enzymes. The second DNase, referred to as “Euglena alkaline DNase,” has a pH optimum of 9.4, requires Ca 2+, a...

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Veröffentlicht in:	Archives of biochemistry and biophysics 1970-05, Vol.138 (1), p.26-31
Hauptverfasser:	Carell, Edgar F., Egan, James M., Pratt, E.A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adaptation, Physiological Animals Calcium - pharmacology Chloroplasts - enzymology Chloroplasts - growth & development Darkness Deoxyribonucleases - analysis Deoxyribonucleases - antagonists & inhibitors Deoxyribonucleases - isolation & purification Deoxyribonucleases - metabolism DNA Euglena - enzymology Euglena - radiation effects Hot Temperature Hydrogen-Ion Concentration Light Nucleic Acid Denaturation Phosphorus Isotopes Radiation Effects Stimulation, Chemical Time Factors
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Zusammenfassung:	Extracts of Euglena contain at least two DNases. One, referred to as “Euglena acid DNase,” has a pH optimum of 4.6, and exhibits enzymic and purification properties similar to DNase II-like enzymes. The second DNase, referred to as “Euglena alkaline DNase,” has a pH optimum of 9.4, requires Ca 2+, and acts preferentially on heat-denatured DNA. Euglena alkaline DNase is either absent, present in low activity, or inhibited in dark-adapted cells; in addition, the activity of this DNase increases during light-induction of chloroplast development. We hypothesize that Euglena alkaline DNase is a chloroplast-related enzyme.
ISSN:	0003-9861 1096-0384
DOI:	10.1016/0003-9861(70)90279-1