CoO x /CeO 2 @C nanopetals derived Cobalt-Cerium Prussian blue with enhanced Dual-Enzyme mimetic activity for detection of ascorbic acid in rat brain during calm/ischemic processes

In this study, we demonstrate that a highly efficient colorimetric sensor prepared from carbon-shielded Co-Ce Prussian blue analog (PBA) nanopetals (CoO /CeO @C) by green chemical deposition method and thermal annealing processes for detection of ascorbic acid (AA) in cerebral microdialysis fluids. The synthesized CoO /CeO @C showed high dual-mimetic activity, i.e., peroxidase- and catalase-like activity, and great catalytic stability. The combination of carbon film and Co-Ce PBA nanopetals (1) greatly enhances the interfacial electron transfer rate of the nanopetals due to excellent electrical conductivity of carbon, and (2) protects nanopetals from acidic chemical environments during the catalytic process, which greatly reduces loss of the catalytic activity of the cobalt-cerium (hydroxide) oxides. Based on the peroxidase-like property of CoO /CeO @C nanopetals, this sensor has a good linear range from 0.1 to 150 μM with a low detection limit of 0.04 μM, i.e., improved sensitivity for AA colorimetric measurement. The developed colorimetric strategy with a green synthetic pathway, catalytic stability and wide linear range confirms the monitoring of AA in brain systems during calm/ischemic processes.