Synergistic effects of silica-enriched bioactive glass and tri-calcium phosphate nanocomposites on BMP2 gene expression for bone repair and regeneration applications

This study focuses on the development of biomaterials for bone regeneration highlighting 59S bioactive glass (59S BG), tri-calcium phosphate (TCP), and their 1:1 composite (59S BG/TCP). The synthesized materials demonstrated excellent properties for bone tissue engineering. Characterization revealed their thermal stability up to 900 °C, as confirmed by thermogravimetric analysis (TGA), while X-ray diffraction (XRD) identified calcium phosphate and silicate phases. Functional groups and chemical bonding were elucidated using Fourier transform infrared spectroscopy (FTIR). The composite exhibited remarkable mechanical properties, with a hardness of 167.87 HV and a strength of 680.52 MPa, indicating its suitability for load-bearing applications. Biological evaluations confirmed promising performance, with in-vitro bioactivity showing apatite formation and reduced XRD peak intensity. Biocompatibility assessments revealed hemolysis below 5 % and a 300 % cell proliferation rate by day three ensuring minimal cytotoxicity and favorable blood compatibility. Protein adsorption studies demonstrated strong interactions with bovine serum albumin (BSA) and lysozyme, supporting protein stability. Additionally, the composite showed enhanced osteogenic potential with elevated BMP2 gene expression indicating its capacity to promote robust bone regeneration. The synergy between 59S BG and TCP underscores the composite's potential as a promising material for effective bone repair and regeneration.