Crystallographic analysis of G-clamp-RNA complex assisted by large scale RNA-binding profile

Crystallographic analysis of G-clamp-RNA complex assisted by large scale RNA-binding profile

We present the X-ray crystal structure of a complex between a G-clamp and an internal loop motif of pre-mir-125a, selected from high affinity RNAs identified in a large-scale RNA-binding profile. This X-ray crystal structure reveals that the G-clamp interacts with three distinct guanine bases, formi...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2024-12
Hauptverfasser: Nagasawa, Ryosuke, Onizuka, Kazumitsu, Kawamura, Karen, Tsuzuki, Kosuke, Murase, Hirotaka, Komatsu, Kaoru R, Miyashita, Emi, Saito, Hirohide, Kondo, Jiro, Nagatsugi, Fumi
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Sprache:eng
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Zusammenfassung:We present the X-ray crystal structure of a complex between a G-clamp and an internal loop motif of pre-mir-125a, selected from high affinity RNAs identified in a large-scale RNA-binding profile. This X-ray crystal structure reveals that the G-clamp interacts with three distinct guanine bases, forming robust bonds through hydrogen bonding and stacking interactions.
ISSN:1364-548X