Hybrid recognition-enabled ratiometric electrochemical sensing of Staphylococcus aureus via in-situ growth of MOF/Ti 3 C 2 T x -MXene and a self-reporting bacterial imprinted polymer
Rapid and effective analysis of foodborne bacteria is crucial for preventing and controlling bacterial infections. Here, we present the synthesis of a self-reporting molecularly imprinted polymer (MIP) as an inner reference probe (IR), and the in-situ growth of metal-organic frameworks on transition...
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| Veröffentlicht in: | Food chemistry 2025-01, Vol.463 (Pt 4), p.141496 |
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| container_issue | Pt 4 |
| container_start_page | 141496 |
| container_title | Food chemistry |
| container_volume | 463 |
| creator | Liu, Yang Meng, Xianzhu Ma, Zhongrui Gu, Huiwen Luo, Xiao Yin, Xiaoli Yi, Hongchao Chen, Ying |
| description | Rapid and effective analysis of foodborne bacteria is crucial for preventing and controlling bacterial infections. Here, we present the synthesis of a self-reporting molecularly imprinted polymer (MIP) as an inner reference probe (IR), and the in-situ growth of metal-organic frameworks on transition metal carbon nitrides (MOF/Ti
C
T
-MXene) as a signaling nanoprobe (SP). These advancements are then applied in a ratiometric electrochemical bioassay for Staphylococcus aureus (S. aureus) using a hybrid recognition mechanism. When S. aureus is present, the aptamer-integrated MIP (MIP@Apt) efficiently captures it, followed by binding with SP to form a sandwich structure. This leads to decreased current response of IR (I
) and increased current intensity of SP (I
), enabling quantification through utilization of the I
to I
ratio. The biosensor shows a wide detection range (10-10
CFU mL
) and low detection limit of 1.2 CFU mL
. Its feasibility for testing complex samples indicates the potential application in food analysis. |
| format | Article |
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C
T
-MXene) as a signaling nanoprobe (SP). These advancements are then applied in a ratiometric electrochemical bioassay for Staphylococcus aureus (S. aureus) using a hybrid recognition mechanism. When S. aureus is present, the aptamer-integrated MIP (MIP@Apt) efficiently captures it, followed by binding with SP to form a sandwich structure. This leads to decreased current response of IR (I
) and increased current intensity of SP (I
), enabling quantification through utilization of the I
to I
ratio. The biosensor shows a wide detection range (10-10
CFU mL
) and low detection limit of 1.2 CFU mL
. Its feasibility for testing complex samples indicates the potential application in food analysis.</description><identifier>EISSN: 1873-7072</identifier><identifier>PMID: 39378721</identifier><language>eng</language><publisher>England</publisher><subject>Biosensing Techniques ; Electrochemical Techniques ; Limit of Detection ; Metal-Organic Frameworks - chemistry ; Molecular Imprinting ; Molecularly Imprinted Polymers - chemistry ; Polymers - chemistry ; Staphylococcus aureus - growth & development ; Staphylococcus aureus - isolation & purification</subject><ispartof>Food chemistry, 2025-01, Vol.463 (Pt 4), p.141496</ispartof><rights>Copyright © 2024 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39378721$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Yang</creatorcontrib><creatorcontrib>Meng, Xianzhu</creatorcontrib><creatorcontrib>Ma, Zhongrui</creatorcontrib><creatorcontrib>Gu, Huiwen</creatorcontrib><creatorcontrib>Luo, Xiao</creatorcontrib><creatorcontrib>Yin, Xiaoli</creatorcontrib><creatorcontrib>Yi, Hongchao</creatorcontrib><creatorcontrib>Chen, Ying</creatorcontrib><title>Hybrid recognition-enabled ratiometric electrochemical sensing of Staphylococcus aureus via in-situ growth of MOF/Ti 3 C 2 T x -MXene and a self-reporting bacterial imprinted polymer</title><title>Food chemistry</title><addtitle>Food Chem</addtitle><description>Rapid and effective analysis of foodborne bacteria is crucial for preventing and controlling bacterial infections. Here, we present the synthesis of a self-reporting molecularly imprinted polymer (MIP) as an inner reference probe (IR), and the in-situ growth of metal-organic frameworks on transition metal carbon nitrides (MOF/Ti
C
T
-MXene) as a signaling nanoprobe (SP). These advancements are then applied in a ratiometric electrochemical bioassay for Staphylococcus aureus (S. aureus) using a hybrid recognition mechanism. When S. aureus is present, the aptamer-integrated MIP (MIP@Apt) efficiently captures it, followed by binding with SP to form a sandwich structure. This leads to decreased current response of IR (I
) and increased current intensity of SP (I
), enabling quantification through utilization of the I
to I
ratio. The biosensor shows a wide detection range (10-10
CFU mL
) and low detection limit of 1.2 CFU mL
. Its feasibility for testing complex samples indicates the potential application in food analysis.</description><subject>Biosensing Techniques</subject><subject>Electrochemical Techniques</subject><subject>Limit of Detection</subject><subject>Metal-Organic Frameworks - chemistry</subject><subject>Molecular Imprinting</subject><subject>Molecularly Imprinted Polymers - chemistry</subject><subject>Polymers - chemistry</subject><subject>Staphylococcus aureus - growth & development</subject><subject>Staphylococcus aureus - isolation & purification</subject><issn>1873-7072</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2025</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFj81OwzAQhC0kREvhFdC-gEUSHxLOFVUvFQdy4FY5ziZZ5J9o7QB5MZ4PV4Izp9GOPs3OXIlt2dRK1kVdbcRtjO9FUVRF2dyIjXpSdVNX5VZ8H9eOqQdGE0ZPiYKX6HVnMXs6nw4TkwG0aBIHM6Ejoy1E9JH8CGGA16TnabXBBGOWCHphzPJBGsjLSGmBkcNnmi7s6eXw2BIo2EMFLXyBPL2hR9C-B51D7SAZ58Dpkt1pk5ApfyM3M_mUO83Brg75TlwP2ka8_9WdeDg8t_ujnJfOYX_OuNO8nv-Gqn-BHwSpYXg</recordid><startdate>20250115</startdate><enddate>20250115</enddate><creator>Liu, Yang</creator><creator>Meng, Xianzhu</creator><creator>Ma, Zhongrui</creator><creator>Gu, Huiwen</creator><creator>Luo, Xiao</creator><creator>Yin, Xiaoli</creator><creator>Yi, Hongchao</creator><creator>Chen, Ying</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>20250115</creationdate><title>Hybrid recognition-enabled ratiometric electrochemical sensing of Staphylococcus aureus via in-situ growth of MOF/Ti 3 C 2 T x -MXene and a self-reporting bacterial imprinted polymer</title><author>Liu, Yang ; Meng, Xianzhu ; Ma, Zhongrui ; Gu, Huiwen ; Luo, Xiao ; Yin, Xiaoli ; Yi, Hongchao ; Chen, Ying</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-pubmed_primary_393787213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2025</creationdate><topic>Biosensing Techniques</topic><topic>Electrochemical Techniques</topic><topic>Limit of Detection</topic><topic>Metal-Organic Frameworks - chemistry</topic><topic>Molecular Imprinting</topic><topic>Molecularly Imprinted Polymers - chemistry</topic><topic>Polymers - chemistry</topic><topic>Staphylococcus aureus - growth & development</topic><topic>Staphylococcus aureus - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Yang</creatorcontrib><creatorcontrib>Meng, Xianzhu</creatorcontrib><creatorcontrib>Ma, Zhongrui</creatorcontrib><creatorcontrib>Gu, Huiwen</creatorcontrib><creatorcontrib>Luo, Xiao</creatorcontrib><creatorcontrib>Yin, Xiaoli</creatorcontrib><creatorcontrib>Yi, Hongchao</creatorcontrib><creatorcontrib>Chen, Ying</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Yang</au><au>Meng, Xianzhu</au><au>Ma, Zhongrui</au><au>Gu, Huiwen</au><au>Luo, Xiao</au><au>Yin, Xiaoli</au><au>Yi, Hongchao</au><au>Chen, Ying</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hybrid recognition-enabled ratiometric electrochemical sensing of Staphylococcus aureus via in-situ growth of MOF/Ti 3 C 2 T x -MXene and a self-reporting bacterial imprinted polymer</atitle><jtitle>Food chemistry</jtitle><addtitle>Food Chem</addtitle><date>2025-01-15</date><risdate>2025</risdate><volume>463</volume><issue>Pt 4</issue><spage>141496</spage><pages>141496-</pages><eissn>1873-7072</eissn><abstract>Rapid and effective analysis of foodborne bacteria is crucial for preventing and controlling bacterial infections. Here, we present the synthesis of a self-reporting molecularly imprinted polymer (MIP) as an inner reference probe (IR), and the in-situ growth of metal-organic frameworks on transition metal carbon nitrides (MOF/Ti
C
T
-MXene) as a signaling nanoprobe (SP). These advancements are then applied in a ratiometric electrochemical bioassay for Staphylococcus aureus (S. aureus) using a hybrid recognition mechanism. When S. aureus is present, the aptamer-integrated MIP (MIP@Apt) efficiently captures it, followed by binding with SP to form a sandwich structure. This leads to decreased current response of IR (I
) and increased current intensity of SP (I
), enabling quantification through utilization of the I
to I
ratio. The biosensor shows a wide detection range (10-10
CFU mL
) and low detection limit of 1.2 CFU mL
. Its feasibility for testing complex samples indicates the potential application in food analysis.</abstract><cop>England</cop><pmid>39378721</pmid></addata></record> |
| fulltext | fulltext |
| identifier | EISSN: 1873-7072 |
| ispartof | Food chemistry, 2025-01, Vol.463 (Pt 4), p.141496 |
| issn | 1873-7072 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Biosensing Techniques Electrochemical Techniques Limit of Detection Metal-Organic Frameworks - chemistry Molecular Imprinting Molecularly Imprinted Polymers - chemistry Polymers - chemistry Staphylococcus aureus - growth & development Staphylococcus aureus - isolation & purification |
| title | Hybrid recognition-enabled ratiometric electrochemical sensing of Staphylococcus aureus via in-situ growth of MOF/Ti 3 C 2 T x -MXene and a self-reporting bacterial imprinted polymer |
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