Hybrid recognition-enabled ratiometric electrochemical sensing of Staphylococcus aureus via in-situ growth of MOF/Ti 3 C 2 T x -MXene and a self-reporting bacterial imprinted polymer

Rapid and effective analysis of foodborne bacteria is crucial for preventing and controlling bacterial infections. Here, we present the synthesis of a self-reporting molecularly imprinted polymer (MIP) as an inner reference probe (IR), and the in-situ growth of metal-organic frameworks on transition...

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Veröffentlicht in:Food chemistry 2025-01, Vol.463 (Pt 4), p.141496
Hauptverfasser: Liu, Yang, Meng, Xianzhu, Ma, Zhongrui, Gu, Huiwen, Luo, Xiao, Yin, Xiaoli, Yi, Hongchao, Chen, Ying
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Sprache:eng
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Zusammenfassung:Rapid and effective analysis of foodborne bacteria is crucial for preventing and controlling bacterial infections. Here, we present the synthesis of a self-reporting molecularly imprinted polymer (MIP) as an inner reference probe (IR), and the in-situ growth of metal-organic frameworks on transition metal carbon nitrides (MOF/Ti C T -MXene) as a signaling nanoprobe (SP). These advancements are then applied in a ratiometric electrochemical bioassay for Staphylococcus aureus (S. aureus) using a hybrid recognition mechanism. When S. aureus is present, the aptamer-integrated MIP (MIP@Apt) efficiently captures it, followed by binding with SP to form a sandwich structure. This leads to decreased current response of IR (I ) and increased current intensity of SP (I ), enabling quantification through utilization of the I to I ratio. The biosensor shows a wide detection range (10-10  CFU mL ) and low detection limit of 1.2 CFU mL . Its feasibility for testing complex samples indicates the potential application in food analysis.
ISSN:1873-7072